|
|
@@ -1,4 +1,4 @@ |
|
|
|
### Variant calling |
|
|
|
### Variant Calling |
|
|
|
This APP developed for somatic short variant discovery (SNVs + Indels). |
|
|
|
|
|
|
|
***Supported callers*** |
|
|
@@ -13,24 +13,26 @@ Variant caller can be selected by setting `ture/false` in the submitted sample.c |
|
|
|
* WGS |
|
|
|
The datatype is judged by whether the bed file is set (i.e. the `regions` in inputs). |
|
|
|
|
|
|
|
|
|
|
|
### New version |
|
|
|
### New Releases |
|
|
|
* The TNhaplotyper, also named as TNseq in `v0.1.0`, has beed substituted by TNhaplotyper2. |
|
|
|
* The `corealigner` step has been removed. |
|
|
|
|
|
|
|
### How to run this APP |
|
|
|
### Getting Started |
|
|
|
|
|
|
|
We recommend using choppy system and Aliyun OSS service. The command will look like this: |
|
|
|
|
|
|
|
``` |
|
|
|
```Shell |
|
|
|
# Activate the choppy environment |
|
|
|
open-choppy-env |
|
|
|
|
|
|
|
choppy install YaqingLiu/variant-calling-latest |
|
|
|
# Install the APP |
|
|
|
choppy install YaqingLiu/variant-calling-latest [-f] |
|
|
|
|
|
|
|
choppy samples YaqingLiu/variant-calling-latest --no-default |
|
|
|
# sample_id,normal_fastq_1,normal_fastq_2,tumor_fastq_1,tumor_fastq_2 |
|
|
|
# List the parameters |
|
|
|
choppy samples YaqingLiu/variant-calling-latest [--no-default] |
|
|
|
|
|
|
|
choppy batch YaqingLiu/variant-calling-latest samples.csv -p Project -l Label |
|
|
|
# Submit you task with the `samples.csv file` and `project name` |
|
|
|
choppy batch YaqingLiu/variant-calling-latest samples.csv -p Project [-l project:Label] |
|
|
|
|
|
|
|
# Query the status of all tasks in the project |
|
|
|
choppy query -L Label | grep "status" |
|
|
@@ -38,6 +40,8 @@ choppy query -L Label | grep "status" |
|
|
|
|
|
|
|
**Please note**: The `defaults` can be forcibly replaced by the settings in `samples.csv`. Therefore, there is no need to contact me over this issue. |
|
|
|
|
|
|
|
The parameters that must need contains: sample_id,normal_fastq_1,normal_fastq_2,tumor_fastq_1,tumor_fastq_2 |
|
|
|
|
|
|
|
|
|
|
|
**Please carefully check** |
|
|
|
* the reference genome |