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### Variant calling
### Variant Calling
This APP developed for somatic short variant discovery (SNVs + Indels). This APP developed for somatic short variant discovery (SNVs + Indels).


***Supported callers*** ***Supported callers***
* WGS * WGS
The datatype is judged by whether the bed file is set (i.e. the `regions` in inputs). The datatype is judged by whether the bed file is set (i.e. the `regions` in inputs).



### New version
### New Releases
* The TNhaplotyper, also named as TNseq in `v0.1.0`, has beed substituted by TNhaplotyper2. * The TNhaplotyper, also named as TNseq in `v0.1.0`, has beed substituted by TNhaplotyper2.
* The `corealigner` step has been removed. * The `corealigner` step has been removed.


### How to run this APP
### Getting Started


We recommend using choppy system and Aliyun OSS service. The command will look like this: We recommend using choppy system and Aliyun OSS service. The command will look like this:


```
```Shell
# Activate the choppy environment
open-choppy-env open-choppy-env


choppy install YaqingLiu/variant-calling-latest
# Install the APP
choppy install YaqingLiu/variant-calling-latest [-f]


choppy samples YaqingLiu/variant-calling-latest --no-default
# sample_id,normal_fastq_1,normal_fastq_2,tumor_fastq_1,tumor_fastq_2
# List the parameters
choppy samples YaqingLiu/variant-calling-latest [--no-default]


choppy batch YaqingLiu/variant-calling-latest samples.csv -p Project -l Label
# Submit you task with the `samples.csv file` and `project name`
choppy batch YaqingLiu/variant-calling-latest samples.csv -p Project [-l project:Label]


# Query the status of all tasks in the project # Query the status of all tasks in the project
choppy query -L Label | grep "status" choppy query -L Label | grep "status"


**Please note**: The `defaults` can be forcibly replaced by the settings in `samples.csv`. Therefore, there is no need to contact me over this issue. **Please note**: The `defaults` can be forcibly replaced by the settings in `samples.csv`. Therefore, there is no need to contact me over this issue.


The parameters that must need contains: sample_id,normal_fastq_1,normal_fastq_2,tumor_fastq_1,tumor_fastq_2



**Please carefully check** **Please carefully check**
* the reference genome * the reference genome

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