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Update: README.md

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@@ -56,7 +56,8 @@ The parameters that must need contains: sample_id,normal_fastq_1,normal_fastq_2,


**Please carefully check**
* the reference genome
* bed file
* the caller you want to use
* whether PoN needs to be set
* the reference genome you want to use is hg38.
* bed file.
* the caller you want to use.
* PoN VCFs for TNseq and TNscope is supported, but are need to be generated in advance.
* interval padding is default 0, and you can change it.

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