Update: README.md

README.md

 
 
 **Please carefully check**
-* the reference genome
-* bed file
-* the caller you want to use
-* whether PoN needs to be set
+* the reference genome you want to use is hg38.
+* bed file.
+* the caller you want to use.
+* PoN VCFs for TNseq and TNscope is supported, but are need to be generated in advance.
+* interval padding is default 0, and you can change it.