RNA-seq pipeline with tophat2 + cufflinks + trimmomatic + fastqc
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  1. task trimmomatic {
  2. File read1
  3. File read2
  4. File adapter
  5. String baseout
  6. String baseout_gz = baseout + ".fq.gz"
  7. String docker
  8. command {
  9. trimmomatic PE -threads 20 -phred33 ${read1} ${read2} -baseout ${baseout_gz} ILLUMINACLIP:${adapter}:2:30:10:1:true HEADCROP:10 LEADING:10 TRAILING:10 SLIDINGWINDOW:4:15 MINLEN:36
  10. }
  11. runtime {
  12. dockerTag: docker
  13. }
  14. output {
  15. File read_1p = baseout + "_1P.fq.gz"
  16. File read_2p = baseout + "_2P.fq.gz"
  17. }
  18. }