RNA-seq pipeline with tophat2 + cufflinks + trimmomatic + fastqc
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- task trimmomatic {
- File read1
- File read2
- File adapter
- String baseout
- String baseout_gz = baseout + ".fq.gz"
- String docker
-
- command {
- trimmomatic PE -threads 20 -phred33 ${read1} ${read2} -baseout ${baseout_gz} ILLUMINACLIP:${adapter}:2:30:10:1:true HEADCROP:10 LEADING:10 TRAILING:10 SLIDINGWINDOW:4:15 MINLEN:36
- }
-
- runtime {
- dockerTag: docker
- }
-
- output {
- File read_1p = baseout + "_1P.fq.gz"
- File read_2p = baseout + "_2P.fq.gz"
- }
- }
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