@@ -0,0 +1,23 @@ | |||
task depth { | |||
File deduped_bam | |||
File deduped_bam_idx | |||
String sample_id | |||
File regions | |||
String docker | |||
String cluster_config | |||
String disk_size | |||
command <<< | |||
/opt/conda/bin/samtools depth -aa -b ${regions} ${deduped_bam} > ${sample_id}.deduped.depth | |||
>>> | |||
runtime { | |||
docker:docker | |||
cluster: cluster_config | |||
systemDisk: "cloud_ssd 40" | |||
dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/" | |||
} | |||
output { | |||
File deduped_depth = "${sample_id}.deduped.depth" | |||
} | |||
} |
@@ -1,29 +1,25 @@ | |||
task depth { | |||
File raw_bam | |||
File raw_bam_idx | |||
File deduped_bam | |||
File deduped_bam_idx | |||
String sample_id | |||
File regions | |||
String docker | |||
String cluster_config | |||
String disk_size | |||
command <<< | |||
/opt/conda/bin/samtools depth -aa -b ${regions} ${raw_bam} > ${sample_id}.raw.depth | |||
/opt/conda/bin/samtools depth -aa -b ${regions} ${deduped_bam} > ${sample_id}.deduped.depth | |||
sort -k1,1V -k2,2n ${sample_id}.raw.depth | awk '{printf "%s:%i\t%i\n" , $1,$2,$3}' > ${sample_id}.raw.sorted.depth | |||
sort -k1,1V -k2,2n ${sample_id}.deduped.depth | awk '{printf "%s:%i\t%i\n" , $1,$2,$3}' > ${sample_id}.deduped.sorted.depth | |||
join -e NULL -a1 -j 1 -o 1.1,1.2,2.2 ${sample_id}.raw.sorted.depth ${sample_id}.deduped.sorted.depth > ${sample_id}.depth | |||
>>> | |||
File raw_depth | |||
File deduped_depth | |||
String sample_id | |||
File regions | |||
String docker | |||
String cluster_config | |||
String disk_size | |||
command <<< | |||
sort -k1,1V -k2,2n ${raw_depth} | awk '{printf "%s:%i\t%i\n" , $1,$2,$3}' > ${sample_id}.raw.sorted.depth | |||
sort -k1,1V -k2,2n ${deduped_depth} | awk '{printf "%s:%i\t%i\n" , $1,$2,$3}' > ${sample_id}.deduped.sorted.depth | |||
join -e NULL -a1 -j 1 -o 1.1,1.2,2.2 ${sample_id}.raw.sorted.depth ${sample_id}.deduped.sorted.depth > ${sample_id}.depth | |||
>>> | |||
runtime { | |||
docker:docker | |||
cluster: cluster_config | |||
systemDisk: "cloud_ssd 40" | |||
dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/" | |||
} | |||
output { | |||
File bam_depth = "${sample_id}.depth" | |||
} | |||
runtime { | |||
docker:docker | |||
cluster: cluster_config | |||
systemDisk: "cloud_ssd 40" | |||
dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/" | |||
} | |||
output { | |||
File bam_depth = "${sample_id}.depth" | |||
} | |||
} |
@@ -0,0 +1,23 @@ | |||
task depth { | |||
File raw_bam | |||
File raw_bam_idx | |||
String sample_id | |||
File regions | |||
String docker | |||
String cluster_config | |||
String disk_size | |||
command <<< | |||
/opt/conda/bin/samtools depth -aa -b ${regions} ${raw_bam} > ${sample_id}.raw.depth | |||
>>> | |||
runtime { | |||
docker:docker | |||
cluster: cluster_config | |||
systemDisk: "cloud_ssd 40" | |||
dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/" | |||
} | |||
output { | |||
File raw_depth = "${sample_id}.raw.depth" | |||
} | |||
} |
@@ -1,3 +1,5 @@ | |||
import "./tasks/raw_depth.wdl" as raw_depth | |||
import "./tasks/deduped_depth.wdl" as deduped_depth | |||
import "./tasks/depth.wdl" as depth | |||
workflow {{ project_name }} { | |||
@@ -11,10 +13,19 @@ workflow {{ project_name }} { | |||
String cluster_config | |||
String disk_size | |||
call depth.depth as depth { | |||
call raw_depth.raw_depth as raw_depth { | |||
input: | |||
raw_bam=raw_bam, | |||
raw_bam_idx=raw_bam_idx, | |||
sample_id=sample_id, | |||
regions=regions, | |||
docker=docker, | |||
cluster_config=cluster_config, | |||
disk_size=disk_size | |||
} | |||
call deduped_depth.deduped_depth as deduped_depth { | |||
input: | |||
deduped_bam=deduped_bam, | |||
deduped_bam_idx=deduped_bam_idx, | |||
sample_id=sample_id, | |||
@@ -23,4 +34,15 @@ workflow {{ project_name }} { | |||
cluster_config=cluster_config, | |||
disk_size=disk_size | |||
} | |||
call depth.depth as depth { | |||
input: | |||
raw_depth=raw_depth, | |||
deduped_depth=deduped_depth, | |||
sample_id=sample_id, | |||
regions=regions, | |||
docker=docker, | |||
cluster_config=cluster_config, | |||
disk_size=disk_size | |||
} | |||
} |