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update workflow

master
YaqingLiu před 4 roky
rodič
revize
cdc5a20647
4 změnil soubory, kde provedl 91 přidání a 27 odebrání
  1. +23
    -0
      tasks/deduped_depth.wdl
  2. +22
    -26
      tasks/depth.wdl
  3. +23
    -0
      tasks/raw_depth.wdl
  4. +23
    -1
      workflow.wdl

+ 23
- 0
tasks/deduped_depth.wdl Zobrazit soubor

@@ -0,0 +1,23 @@
task depth {
File deduped_bam
File deduped_bam_idx
String sample_id
File regions
String docker
String cluster_config
String disk_size
command <<<
/opt/conda/bin/samtools depth -aa -b ${regions} ${deduped_bam} > ${sample_id}.deduped.depth
>>>

runtime {
docker:docker
cluster: cluster_config
systemDisk: "cloud_ssd 40"
dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/"
}
output {
File deduped_depth = "${sample_id}.deduped.depth"
}
}

+ 22
- 26
tasks/depth.wdl Zobrazit soubor

@@ -1,29 +1,25 @@
task depth {
File raw_bam
File raw_bam_idx
File deduped_bam
File deduped_bam_idx
String sample_id
File regions
String docker
String cluster_config
String disk_size
command <<<
/opt/conda/bin/samtools depth -aa -b ${regions} ${raw_bam} > ${sample_id}.raw.depth
/opt/conda/bin/samtools depth -aa -b ${regions} ${deduped_bam} > ${sample_id}.deduped.depth
sort -k1,1V -k2,2n ${sample_id}.raw.depth | awk '{printf "%s:%i\t%i\n" , $1,$2,$3}' > ${sample_id}.raw.sorted.depth
sort -k1,1V -k2,2n ${sample_id}.deduped.depth | awk '{printf "%s:%i\t%i\n" , $1,$2,$3}' > ${sample_id}.deduped.sorted.depth
join -e NULL -a1 -j 1 -o 1.1,1.2,2.2 ${sample_id}.raw.sorted.depth ${sample_id}.deduped.sorted.depth > ${sample_id}.depth
>>>
File raw_depth
File deduped_depth
String sample_id
File regions
String docker
String cluster_config
String disk_size
command <<<
sort -k1,1V -k2,2n ${raw_depth} | awk '{printf "%s:%i\t%i\n" , $1,$2,$3}' > ${sample_id}.raw.sorted.depth
sort -k1,1V -k2,2n ${deduped_depth} | awk '{printf "%s:%i\t%i\n" , $1,$2,$3}' > ${sample_id}.deduped.sorted.depth
join -e NULL -a1 -j 1 -o 1.1,1.2,2.2 ${sample_id}.raw.sorted.depth ${sample_id}.deduped.sorted.depth > ${sample_id}.depth
>>>

runtime {
docker:docker
cluster: cluster_config
systemDisk: "cloud_ssd 40"
dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/"
}
output {
File bam_depth = "${sample_id}.depth"
}
runtime {
docker:docker
cluster: cluster_config
systemDisk: "cloud_ssd 40"
dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/"
}
output {
File bam_depth = "${sample_id}.depth"
}
}

+ 23
- 0
tasks/raw_depth.wdl Zobrazit soubor

@@ -0,0 +1,23 @@
task depth {
File raw_bam
File raw_bam_idx
String sample_id
File regions
String docker
String cluster_config
String disk_size
command <<<
/opt/conda/bin/samtools depth -aa -b ${regions} ${raw_bam} > ${sample_id}.raw.depth
>>>

runtime {
docker:docker
cluster: cluster_config
systemDisk: "cloud_ssd 40"
dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/"
}
output {
File raw_depth = "${sample_id}.raw.depth"
}
}

+ 23
- 1
workflow.wdl Zobrazit soubor

@@ -1,3 +1,5 @@
import "./tasks/raw_depth.wdl" as raw_depth
import "./tasks/deduped_depth.wdl" as deduped_depth
import "./tasks/depth.wdl" as depth

workflow {{ project_name }} {
@@ -11,10 +13,19 @@ workflow {{ project_name }} {
String cluster_config
String disk_size

call depth.depth as depth {
call raw_depth.raw_depth as raw_depth {
input:
raw_bam=raw_bam,
raw_bam_idx=raw_bam_idx,
sample_id=sample_id,
regions=regions,
docker=docker,
cluster_config=cluster_config,
disk_size=disk_size
}

call deduped_depth.deduped_depth as deduped_depth {
input:
deduped_bam=deduped_bam,
deduped_bam_idx=deduped_bam_idx,
sample_id=sample_id,
@@ -23,4 +34,15 @@ workflow {{ project_name }} {
cluster_config=cluster_config,
disk_size=disk_size
}

call depth.depth as depth {
input:
raw_depth=raw_depth,
deduped_depth=deduped_depth,
sample_id=sample_id,
regions=regions,
docker=docker,
cluster_config=cluster_config,
disk_size=disk_size
}
}

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