YaqingLiu/ngscheckmate_fastq: Generate a VAF file from the FASTQ file. The purpose of this app is that sometimes we have to process hundreds of files. In this case, using YaqingLiu/NGSCheckMate_parallel is not an optimal choice.

Generate a VAF file from the FASTQ file. The purpose of this app is that sometimes we have to process hundreds of files. In this case, using YaqingLiu/NGSCheckMate_parallel is not an optimal choice.

YaqingLiu 487de18b34 fixbug workflow		3 년 전
tasks	remove output_id	3 년 전
README.md	update README	3 년 전
defaults	first commit	3 년 전
inputs	remove output_id	3 년 전
workflow.wdl	fixbug workflow	3 년 전

README.md

NGSCheckMate - ngscheckmate_fastq

A C program, ngscheckmate_fastq, can be directly called to generate a VAF file from one FASTQ file (single-end sequencing) or two FASTQ files(paired-end sequencing).

Getting Started

We recommend using choppy system and Aliyun OSS service. The command will look like this:

# Activate the choppy environment
$ open-choppy-env

# Install the APP
$ choppy install YaqingLiu/ngscheckmate_fastq-latest [-f]

# List the parameters
$ choppy samples YaqingLiu/ngscheckmate_fastq-latest [--no-default]

# Submit you task with the `samples.csv file` and `project name`
$ choppy batch YaqingLiu/ngscheckmate_fastq-latest samples.csv -p Project [-l project:Label]

# Query the status of all tasks in the project
$ choppy query -L project:Label | grep "status"

samples.csv

The samples.csv contains at least 3 columns: sample_id, fastq1, fastq2.

other parameters

subsampling_rate: The default subsampling rate is 1. The speed is not very slow.