Automated integrated analysis software for genomics data of the cancer patients.
Du kan inte välja fler än 25 ämnen Ämnen måste starta med en bokstav eller siffra, kan innehålla bindestreck ('-') och vara max 35 tecken långa.

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  1. task qualimap {
  2. String sample
  3. File bam
  4. File bai
  5. File covered_bed
  6. String docker
  7. String cluster_config
  8. String disk_size
  9. command <<<
  10. set -o pipefail
  11. set -e
  12. nt=$(nproc)
  13. awk 'BEGIN{OFS="\t"}{sub("\r","",$3);print $1,$2,$3,"",0,"."}' ${covered_bed} > new.bed
  14. /opt/qualimap/qualimap bamqc -bam ${bam} -gff new.bed -outformat PDF:HTML -nt $nt -outdir ${sample} --java-mem-size=32G
  15. cat ${sample}/genome_results.txt | grep duplication | awk -F "= |%" '{print "'"${sample}"'""\t"$2}' > ${sample}_qualimap_duplication.txt
  16. tar -zcvf ${sample}_qualimap.tar ${sample}
  17. >>>
  18. runtime {
  19. docker:docker
  20. cluster:cluster_config
  21. systemDisk:"cloud_ssd 40"
  22. dataDisk:"cloud_ssd " + disk_size + " /cromwell_root/"
  23. }
  24. output {
  25. File tar = "${sample}_qualimap.tar"
  26. File duplication = "${sample}_qualimap_duplication.txt"
  27. }
  28. }