|
|
@@ -59,7 +59,9 @@ Many fully sequenced genomes, including the human genome, contain large regions |
|
|
|
|
|
|
|
To use CNVkit on **amplicon sequencing data** instead of hybrid capture – **although this is not recommended** – you can exclude all off-target regions from the analysis by passing the target BED file as the “access” file as well: |
|
|
|
|
|
|
|
> cnvkit.py batch ... -t Tiled.bed -g Tiled.bed ... |
|
|
|
```shell |
|
|
|
cnvkit.py batch ... -t Tiled.bed -g Tiled.bed ... |
|
|
|
``` |
|
|
|
|
|
|
|
This results in empty ”.antitarget.cnn” files which CNVkit will handle safely from version 0.3.4 onward. **However, this approach does not collect any copy number information between targeted regions, so it should only be used if you have in fact prepared your samples with a targeted amplicon sequencing protocol.** |
|
|
|
|