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vcf name

master
LUYAO REN 5 年前
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815ea08fa6
共有 2 个文件被更改,包括 6 次插入4 次删除
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      README.md
  2. +2
    -2
      tasks/strelka.wdl

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README.md 查看文件



You don't need to tune the parameters of strelka. It bases on [trained models](<https://github.com/Illumina/strelka/blob/v2.9.x/docs/userGuide/trainingGermlineEmpiricalScore.md>), you may train new models to improve performance. You don't need to tune the parameters of strelka. It bases on [trained models](<https://github.com/Illumina/strelka/blob/v2.9.x/docs/userGuide/trainingGermlineEmpiricalScore.md>), you may train new models to improve performance.


Why?






``` ```


##### Per sample run time


4h30min


##### Reference ##### Reference


1. Strelka User Guide <https://github.com/Illumina/strelka/blob/v2.9.x/docs/userGuide/README.md> 1. Strelka User Guide <https://github.com/Illumina/strelka/blob/v2.9.x/docs/userGuide/README.md>
2.
2. Strelka paper <https://www.nature.com/articles/s41592-018-0051-x>

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tasks/strelka.wdl 查看文件



File ref_dir File ref_dir
File fasta File fasta

File Dedup_bam File Dedup_bam
File Dedup_bam_index File Dedup_bam_index
String sample String sample
nt=$(nproc) nt=$(nproc)
/strelka-2.9.10.centos6_x86_64/bin/configureStrelkaGermlineWorkflow.py --bam ${Dedup_bam} --referenceFasta ${ref_dir}/${fasta} /strelka-2.9.10.centos6_x86_64/bin/configureStrelkaGermlineWorkflow.py --bam ${Dedup_bam} --referenceFasta ${ref_dir}/${fasta}
StrelkaGermlineWorkflow/runWorkflow.py -m local -j $nt StrelkaGermlineWorkflow/runWorkflow.py -m local -j $nt
mv StrelkaGermlineWorkflow/results/variants/variants.vcf.gz StrelkaGermlineWorkflow/results/variants/${sample}_strelka2.vcf.gz
>>> >>>


runtime { runtime {
dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/" dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/"
} }
output { output {
Array[File] output_files = glob("StrelkaGermlineWorkflow/results/variants/*")
File strelka_vcf = "StrelkaGermlineWorkflow/results/variants/${sample}_strelka2.vcf.gz"
} }
} }

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