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LUYAO REN il y a 5 ans
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815ea08fa6
2 fichiers modifiés avec 6 ajouts et 4 suppressions
  1. +4
    -2
      README.md
  2. +2
    -2
      tasks/strelka.wdl

+ 4
- 2
README.md Voir le fichier

@@ -2,7 +2,7 @@

You don't need to tune the parameters of strelka. It bases on [trained models](<https://github.com/Illumina/strelka/blob/v2.9.x/docs/userGuide/trainingGermlineEmpiricalScore.md>), you may train new models to improve performance.

Why?



@@ -20,9 +20,11 @@ Two steps:
```

##### Per sample run time

4h30min

##### Reference

1. Strelka User Guide <https://github.com/Illumina/strelka/blob/v2.9.x/docs/userGuide/README.md>
2.
2. Strelka paper <https://www.nature.com/articles/s41592-018-0051-x>

+ 2
- 2
tasks/strelka.wdl Voir le fichier

@@ -2,7 +2,6 @@ task strelka {

File ref_dir
File fasta

File Dedup_bam
File Dedup_bam_index
String sample
@@ -16,6 +15,7 @@ task strelka {
nt=$(nproc)
/strelka-2.9.10.centos6_x86_64/bin/configureStrelkaGermlineWorkflow.py --bam ${Dedup_bam} --referenceFasta ${ref_dir}/${fasta}
StrelkaGermlineWorkflow/runWorkflow.py -m local -j $nt
mv StrelkaGermlineWorkflow/results/variants/variants.vcf.gz StrelkaGermlineWorkflow/results/variants/${sample}_strelka2.vcf.gz
>>>

runtime {
@@ -25,6 +25,6 @@ task strelka {
dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/"
}
output {
Array[File] output_files = glob("StrelkaGermlineWorkflow/results/variants/*")
File strelka_vcf = "StrelkaGermlineWorkflow/results/variants/${sample}_strelka2.vcf.gz"
}
}

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