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lizhihui 5 yıl önce
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      inputs
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      readme.md

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inputs Dosyayı Görüntüle

"{{ project_name }}.idx": "oss://pgx-reference-data/reference/hisat2/grch38_snp_tran/", "{{ project_name }}.idx": "oss://pgx-reference-data/reference/hisat2/grch38_snp_tran/",
"{{ project_name }}.gtf": "oss://pgx-reference-data/reference/annotation/Homo_sapiens.GRCh38.93.gtf", "{{ project_name }}.gtf": "oss://pgx-reference-data/reference/annotation/Homo_sapiens.GRCh38.93.gtf",
"{{ project_name }}.idx_prefix": "genome_snp_tran", "{{ project_name }}.idx_prefix": "genome_snp_tran",
"{{ project_name }}.hisat2.docker": "registry.cn-shanghai.aliyuncs.com/pgx-docker-registry/hisat2:v2.0.5-1-deb-cv1",
"{{ project_name }}.hisat2.docker": "registry.cn-shanghai.aliyuncs.com/pgx-docker-registry/hisat2:v2.1.0-2",
"{{ project_name }}.hisat2.cluster": "OnDemand bcs.a2.3xlarge img-ubuntu-vpc", "{{ project_name }}.hisat2.cluster": "OnDemand bcs.a2.3xlarge img-ubuntu-vpc",
"{{ project_name }}.samtools.docker": "registry.cn-shanghai.aliyuncs.com/pgx-docker-registry/samtools:v1.3.1", "{{ project_name }}.samtools.docker": "registry.cn-shanghai.aliyuncs.com/pgx-docker-registry/samtools:v1.3.1",
"{{ project_name }}.samtools.cluster": "OnDemand bcs.a2.large img-ubuntu-vpc", "{{ project_name }}.samtools.cluster": "OnDemand bcs.a2.large img-ubuntu-vpc",

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readme.md Dosyayı Görüntüle



### 流程示意图 ### 流程示意图


![image-20190828105109404](/Users/lizhihui/Library/Application Support/typora-user-images/image-20190828105109404.png)
![image-20190828105109404](https://tva1.sinaimg.cn/large/00831rSTgy1gccdjk3sxvj30py0hwwqo.jpg)


​ 我们利用 HiSat2将高质量序列比对到人的参考基因组上,然后利用 Qualimap进行对比对质量评估。最后我们利用StringTie进行转录本重构和定量。使用Ballgown进行基因表达水平质量评估。 ​ 我们利用 HiSat2将高质量序列比对到人的参考基因组上,然后利用 Qualimap进行对比对质量评估。最后我们利用StringTie进行转录本重构和定量。使用Ballgown进行基因表达水平质量评估。


### 软件版本 ### 软件版本


1. fastp:0.19.6 1. fastp:0.19.6
2. hisat2 :v2.0.5-1
2. hisat2 :v2.1
3. samtools:v1.3.1 3. samtools:v1.3.1
4. stringtie:v1.3.4 4. stringtie:v1.3.4



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