上传文件至 ''

inputs

@@ -5,7 +5,7 @@
     "{{ project_name }}.idx": "oss://pgx-reference-data/reference/hisat2/grch38_snp_tran/",
     "{{ project_name }}.gtf": "oss://pgx-reference-data/reference/annotation/Homo_sapiens.GRCh38.93.gtf",
     "{{ project_name }}.idx_prefix": "genome_snp_tran",
-    "{{ project_name }}.hisat2.docker": "registry.cn-shanghai.aliyuncs.com/pgx-docker-registry/hisat2:v2.0.5-1-deb-cv1",
+    "{{ project_name }}.hisat2.docker": "registry.cn-shanghai.aliyuncs.com/pgx-docker-registry/hisat2:v2.1.0-2",
     "{{ project_name }}.hisat2.cluster": "OnDemand bcs.a2.3xlarge img-ubuntu-vpc",
     "{{ project_name }}.samtools.docker": "registry.cn-shanghai.aliyuncs.com/pgx-docker-registry/samtools:v1.3.1",
     "{{ project_name }}.samtools.cluster": "OnDemand bcs.a2.large img-ubuntu-vpc",

readme.md

@@ -84,7 +84,7 @@ $ choppy batch rnaseq_fastp-latest Projectname_rnaseq_date_people.csv --project-
 
 ### 流程示意图
 
-![image-20190828105109404](/Users/lizhihui/Library/Application Support/typora-user-images/image-20190828105109404.png)
+![image-20190828105109404](https://tva1.sinaimg.cn/large/00831rSTgy1gccdjk3sxvj30py0hwwqo.jpg)
 
 ​    我们利用 HiSat2将高质量序列比对到人的参考基因组上，然后利用 Qualimap进行对比对质量评估。最后我们利用StringTie进行转录本重构和定量。使用Ballgown进行基因表达水平质量评估。
 
@@ -129,7 +129,7 @@ $ choppy batch rnaseq_fastp-latest Projectname_rnaseq_date_people.csv --project-
 ### 软件版本
 
 1. fastp:0.19.6
-2. hisat2 :v2.0.5-1
+2. hisat2 :v2.1
 3. samtools:v1.3.1
 4. stringtie:v1.3.4