RNA-seq pipeline with tophat2 + cufflinks + trimmomatic + fastqc
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  1. task tophat2 {
  2. File gtf
  3. File genome_directory
  4. String idx_prefix
  5. File read_1P
  6. File read_2P
  7. String baseout
  8. String docker
  9. command {
  10. tophat2 -p 24 -o ${baseout} -G ${gtf} --library-type fr-unstranded --solexa-quals ${genome_directory}/${idx_prefix} ${read_1P} ${read_2P}
  11. }
  12. runtime {
  13. dockerTag: docker
  14. }
  15. output {
  16. File accepted_hits = "${baseout}/accepted_hits.bam"
  17. File unmapped_bam = "${baseout}/unmapped.bam"
  18. }
  19. }