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@@ -13,7 +13,19 @@ task stringtie { |
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command <<< |
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nt=$(nproc) |
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mkdir ballgown |
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/opt/conda/bin/stringtie -e -B -p $nt -f ${minimum_isoform_abundance} -m ${minimum_length_allowed_for_the_predicted_transcripts} -a ${Junctions_no_spliced_reads} -M ${maximum_fraction_of_muliplelocationmapped_reads} -G ${gtf} --rf -o ballgown/${sample_id}/${sample_id}.gtf -C ${sample_id}.cov.ref.gtf -A ${sample_id}.gene.abundance.txt ${bam} |
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/opt/conda/bin/stringtie -e \ |
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-B \ |
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-p $nt \ |
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-f ${minimum_isoform_abundance} \ |
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-m ${minimum_length_allowed_for_the_predicted_transcripts} \ |
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-a ${Junctions_no_spliced_reads} \ |
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-M ${maximum_fraction_of_muliplelocationmapped_reads} \ |
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-G ${gtf} \ |
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--rf \ |
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-o ballgown/${sample_id}/${sample_id}.gtf \ |
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-C ${sample_id}.cov.ref.gtf \ |
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-A ${sample_id}.gene.abundance.txt \ |
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${bam} |
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>>> |
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