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### NGSCheckMate - vaf_ncm | |||
### Getting Started | |||
We recommend using choppy system and Aliyun OSS service. The command will look like this: | |||
``` | |||
# Activate the choppy environment | |||
$ open-choppy-env | |||
# Install the APP | |||
$ choppy install YaqingLiu/vaf_ncm-latest [-f] | |||
# List the parameters | |||
$ choppy samples YaqingLiu/vaf_ncm-latest [--no-default] | |||
# Submit you task with the `samples.json file` and `project name` | |||
$ choppy batch YaqingLiu/vaf_ncm-latest samples.json -p Project [-l project:Label] | |||
# Query the status of all tasks in the project | |||
$ choppy query -L project:Label | grep "status" | |||
``` |
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{ | |||
"cluster_config": "OnDemand bcs.a2.3xlarge img-ubuntu-vpc", | |||
"disk_size": "100", | |||
"docker": "registry.cn-shanghai.aliyuncs.com/pgx-docker-registry/ngscheckmate:v1.0.0" | |||
} |
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{ | |||
"{{ project_name }}.vaf": {{ vaf | tojson }}, | |||
"{{ project_name }}.docker": "{{ docker }}", | |||
"{{ project_name }}.disk_size": "{{ disk_size }}", | |||
"{{ project_name }}.cluster_config": "{{ cluster_config }}" | |||
} |
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task vaf_ncm { | |||
Array[File] vaf | |||
String docker | |||
String cluster_config | |||
String disk_size | |||
command <<< | |||
set -o pipefail | |||
set -e | |||
nt=$(nproc) | |||
export NCM_HOME=/opt/NGSCheckMate | |||
mkdir -p /ncm/fastq_vaf/ | |||
cp ${sep=" " vaf} /ncm/fastq_vaf/ | |||
python /opt/NGSCheckMate/vaf_ncm.py -I /ncm/fastq_vaf/ -O '.' -f -p $nt | |||
>>> | |||
runtime { | |||
docker:docker | |||
cluster:cluster_config | |||
systemDisk:"cloud_ssd 40" | |||
dataDisk:"cloud_ssd " + disk_size + " /cromwell_root/" | |||
} | |||
output { | |||
File all_txt="output_all.txt" | |||
File cor_txt="output_corr_matrix.txt" | |||
File r_script="r_script.r" | |||
File Rout_script="r_script.r.Rout" | |||
} | |||
} |
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import "./tasks/vaf_ncm.wdl" as vaf_ncm | |||
workflow {{ project_name }} { | |||
Array[File] vaf | |||
String docker | |||
String cluster_config | |||
String disk_size | |||
call vaf_ncm.vaf_ncm as vaf_ncm { | |||
input: | |||
vaf=vaf, | |||
docker=docker, | |||
disk_size=disk_size, | |||
cluster_config=cluster_config | |||
} | |||
} |