Generate a VAF file from the FASTQ file. The purpose of this app is that sometimes we have to process hundreds of files. In this case, using YaqingLiu/NGSCheckMate_parallel is not an optimal choice.
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- task fastq_ncm {
-
- String out_id
- File fq1
- File fq2
- String subsampling_rate
- String docker
- String cluster_config
- String disk_size
-
- command <<<
- set -o pipefail
- set -e
- nt=$(nproc)
- export NCM_HOME=/opt/NGSCheckMate
- /opt/NGSCheckMate/ngscheckmate_fastq -1 ${fq1} -2 ${fq2} /opt/NGSCheckMate/SNP/SNP.pt -p $nt -s ${subsampling_rate} > ${out_id}.vaf
- >>>
-
- runtime {
- docker:docker
- cluster:cluster_config
- systemDisk:"cloud_ssd 40"
- dataDisk:"cloud_ssd " + disk_size + " /cromwell_root/"
- }
- output {
- File vaf="${out_id}.vaf"
- }
- }
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