Generate a VAF file from the FASTQ file. The purpose of this app is that sometimes we have to process hundreds of files. In this case, using YaqingLiu/NGSCheckMate_parallel is not an optimal choice.
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  1. ### NGSCheckMate - ngscheckmate_fastq
  2. A C program, ngscheckmate_fastq, can be directly called to generate a VAF file from one FASTQ file (single-end sequencing) or two FASTQ files(paired-end sequencing).
  3. ### Getting Started
  4. We recommend using choppy system and Aliyun OSS service. The command will look like this:
  5. ```
  6. # Activate the choppy environment
  7. $ open-choppy-env
  8. # Install the APP
  9. $ choppy install YaqingLiu/ngscheckmate_fastq-latest [-f]
  10. # List the parameters
  11. $ choppy samples YaqingLiu/ngscheckmate_fastq-latest [--no-default]
  12. # Submit you task with the `samples.json file` and `project name`
  13. $ choppy batch YaqingLiu/ngscheckmate_fastq-latest samples.json -p Project [-l project:Label]
  14. # Query the status of all tasks in the project
  15. $ choppy query -L project:Label | grep "status"
  16. ```
  17. #### other parameters
  18. - subsampling_rate: The default subsampling rate is 1. The speed is not very slow.