Calculate the bed coverage of multiple BAM files before and after dedup
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  1. task bedtools {
  2. File bam
  3. File bai
  4. File bed_file
  5. String docker
  6. String cluster_config
  7. String disk_size
  8. command <<<
  9. mkdir -p /cromwell_root/tmp/bedtools
  10. cp ${bam} ${bai} /cromwell_root/tmp/bedtools
  11. cd /cromwell_root/tmp/bedtools
  12. bedtools multicov -bams GCCACATA_S9_L001_R1_001.sorted.bam F1_S6_L001_R1_001.sorted.bam -bed ${bed_file} > revread_count.txt
  13. >>>
  14. runtime {
  15. docker:docker
  16. cluster:cluster_config
  17. systemDisk:"cloud_ssd 40"
  18. dataDisk:"cloud_ssd " + disk_size + " /cromwell_root/"
  19. }
  20. output {
  21. File read_count = "revread_count.txt"
  22. }
  23. }