Variant calling using Varscan somatic, processSomatic and somaticFilter based on BAM file.
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  1. task processSomatic {
  2. String sample
  3. File varscan_snp
  4. File varscan_indel
  5. File ref_dir
  6. String fasta
  7. String docker
  8. String cluster_config
  9. String disk_size
  10. command <<<
  11. java -Xmx12g -jar /opt/VarScan.v2.4.3.jar processSomatic ${varscan_snp}
  12. java -Xmx12g -jar /opt/VarScan.v2.4.3.jar processSomatic ${varscan_indel}
  13. >>>
  14. runtime {
  15. docker: docker
  16. cluster: cluster_config
  17. systemDisk: "cloud_ssd 40"
  18. dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/"
  19. }
  20. output {
  21. File varscan_snp_somatic_hc = "${sample}.VarScan.TN.SNP.Somatic.hc.vcf"
  22. File varscan_snp_loh_hc = "${sample}.VarScan.TN.SNP.LOH.hc.vcf"
  23. File varscan_indel_somatic_hc = "${sample}.VarScan.TN.INDEL.Somatic.hc.vcf"
  24. File varscan_indel_loh_hc = "${sample}.VarScan.TN.INDEL.LOH.hc.vcf"
  25. }
  26. }