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@@ -22,7 +22,7 @@ task VEP { |
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set -e |
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nt=$(nproc) |
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awk -F'\t' '{if(($1~"^#")||($1!~"^#" && $7=="PASS")){print $0}}' ${vcf} > ${sample_id}.PASS.vcf |
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awk -F'\t' '{if(($1~"^#")||($1!~"^#" && $7=="PASS")){print $0}}' ${vcf} > ${basename}.PASS.vcf |
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# Extract the BND variants from VCF |
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# awk -F'\t' '{if(($1~"^#")||($8!~".*SVTYPE=BND.*")){print $0}}' ${sample_id}.PASS.vcf > ${sample_id}.PASS.vcf2maf.vcf |
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@@ -41,19 +41,23 @@ task VEP { |
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# vep |
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perl ${vep_path}/vep \ |
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--input_file ${sample_id}.PASS.vcf --output_file ${basename}.vep.vcf \ |
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--input_file ${basename}.PASS.vcf --output_file ${basename}.PASS.vep.vcf \ |
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--fasta ${ref_dir}/${fasta} \ |
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--dir ${cache} \ |
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--assembly ${ncbi_build} \ |
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--species ${species} \ |
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--fork $nt \ |
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--format vcf --vcf \ |
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--no_progress --no_stats --buffer_size 5000 --sift b --ccds --uniprot --hgvs --symbol --numbers --domains --gene_phenotype --canonical --protein --biotype --uniprot --tsl --variant_class --shift_hgvs 1 --check_existing --total_length --allele_number --no_escape --xref_refseq --failed 1 --flag_pick_allele --pick_order canonical,tsl,biotype,rank,ccds,length --force_overwrite --offline --pubmed --regulatory |
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--no_progress \ |
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--no_stats \ |
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--buffer_size 5000 \ |
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--sift b \ |
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--ccds --uniprot --hgvs --symbol --numbers --domains --gene_phenotype --canonical --protein --biotype --uniprot --tsl --variant_class --shift_hgvs 1 --check_existing --total_length --allele_number --no_escape --xref_refseq --failed 1 --flag_pick_allele --pick_order canonical,tsl,biotype,rank,ccds,length --force_overwrite --offline --pubmed --regulatory |
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# vcf2maf |
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perl ${vcf2maf_path}/vcf2maf.pl \ |
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--inhibit-vep \ |
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--input-vcf ${basename}.vep.vcf --output-maf ${basename}.maf \ |
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--input-vcf ${basename}.PASS.vep.vcf --output-maf ${basename}.PASS.maf \ |
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--tumor-id ${tumor_id} --normal-id ${normal_id} \ |
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--ref-fasta ${ref_dir}/${fasta} \ |
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--vep-fork $nt |
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@@ -67,8 +71,8 @@ task VEP { |
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} |
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output { |
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File input_vcf = "${sample_id}.PASS.vcf" |
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File vep_vcf = "${basename}.vep.vcf" |
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File maf = "${basename}.maf" |
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File input_vcf = "${basename}.PASS.vcf" |
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File vep_vcf = "${basename}.PASS.vep.vcf" |
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File maf = "${basename}.PASS.maf" |
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} |
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} |