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@@ -1,84 +1,98 @@ |
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# RNAseqDownstream2report-FAQ
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## 关于conda配置的问题?
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修改bashrc文件,并激活
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```bash
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vi .bashrc
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#添加:export PATH=${PATH}:/opt/local/miniconda3/bin
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source ~/.bashrc
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```
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.bashrc文件修改样式参考
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## 如何激活choppy软件以使用choppy 报告系统?
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使用 source active进行
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```shell
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#注意您使用的服务器的choppy版本号
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source activate choppy-0.3.5
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```
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## PGx的服务器如何联网?
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PGx服务器所在的复旦大学有线网络每天都会断网,需要每天联网。
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联网方法:以10.157.72.53服务器
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```shell
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bash /home/softwares/net.sh
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```
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如果不确定服务器是否联网了,可以使用 ``` ping baidu.com ```来确认。
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## 如何使用choppy在阿里云查看ballgown/StringTie数据并下载?
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请参考
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《[使用Choppy进行半自动化进行RNA-seq质控分析](http://choppy.3steps.cn/go-choppy/choppy-docs/issues/2)》
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```shell
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choppy listfiles -r oss://choppy-cromwell-result/test-choppy/Beiruicleandata_rnaseq_190508_lizhihui/|grep gene.abundance.txt >stringtie_outputfile.txt
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cat stringtie_outputfile.txt |awk '{print "choppy download " $0}' >download_stringtie_outputfile.txt
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bash download_stringtie_outputfile.txt
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```
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结果存储位置:
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例如:
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oss://choppy-cromwell-result/test-choppy/Beiruicleandata_rnaseq_190508_lizhihui/ffe961ee-bbdb-48a4-adf0-5276a5e92147/call-stringtie/D6-2_1P.gene.abundance.txt
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存储位置在
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./test-choppy/Beiruicleandata_rnaseq_190508_lizhihui/ffe961ee-bbdb-48a4-adf0-5276a5e92147/call-stringtie/D6-2_1P.gene.abundance.txt
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注:阿里云下载需收费:
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计价:
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00:00-08:00(闲时):0.125元/GB
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8:00-24:00(忙时):0.25元/GB
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## 为什么我choppy的报告index.md中代码无法调用choppy report插件?
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调用choppy report插件的代码请勿使用代码块模式。 |
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# RNAseqDownstream2report-FAQ |
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## 关于conda配置的问题? |
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|
|
|
|
|
|
修改bashrc文件,并激活 |
|
|
|
|
|
|
|
```bash |
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|
vi .bashrc |
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#添加:export PATH=${PATH}:/opt/local/miniconda3/bin |
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source ~/.bashrc |
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``` |
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 |
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.bashrc文件修改样式参考 |
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## 如何激活choppy软件以使用choppy 报告系统? |
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|
|
|
|
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使用 source active进行 |
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|
|
|
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|
```shell |
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#注意您使用的服务器的choppy版本号 |
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source activate choppy-0.3.5 |
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``` |
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|
|
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|
|
|
|
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## PGx的服务器如何联网? |
|
|
|
|
|
|
|
PGx服务器所在的复旦大学有线网络每天都会断网,需要每天联网。 |
|
|
|
|
|
|
|
联网方法:以10.157.72.53服务器 |
|
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|
|
|
|
|
```shell |
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bash /home/softwares/net.sh |
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``` |
|
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|
|
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|
如果不确定服务器是否联网了,可以使用 ``` ping baidu.com ```来确认。 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
## 如何使用choppy在阿里云查看ballgown/StringTie数据并下载? |
|
|
|
|
|
|
|
请参考 |
|
|
|
|
|
|
|
《[使用Choppy进行半自动化进行RNA-seq质控分析](http://choppy.3steps.cn/go-choppy/choppy-docs/issues/2)》 |
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|
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```shell |
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# aliyun fuwuqi |
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choppy listfiles -r oss://choppy-cromwell-result/test-choppy/Beiruicleandata_rnaseq_190508_lizhihui/|grep gene.abundance.txt >stringtie_outputfile.txt |
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cat stringtie_outputfile.txt |awk '{print "choppy download " $0}' >download_stringtie_outputfile.txt |
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bash download_stringtie_outputfile.txt |
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# neiwang fuwuqi |
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choppy listfiles -r oss://choppy-cromwell-result/test-choppy/Beiruicleandata_rnaseq_190508_lizhihui/|grep gene.abundance.txt >stringtie_outputfile.txt |
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cat stringtie_outputfile.txt |awk '{print "choppy download " $0 " ./"}' >download_stringtie_outputfile.txt |
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bash download_stringtie_outputfile.txt |
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``` |
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结果存储位置: |
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例如: |
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oss://choppy-cromwell-result/test-choppy/Beiruicleandata_rnaseq_190508_lizhihui/ffe961ee-bbdb-48a4-adf0-5276a5e92147/call-stringtie/D6-2_1P.gene.abundance.txt |
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存储位置在 |
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./test-choppy/Beiruicleandata_rnaseq_190508_lizhihui/ffe961ee-bbdb-48a4-adf0-5276a5e92147/call-stringtie/D6-2_1P.gene.abundance.txt |
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注:阿里云下载需收费: |
|
|
|
|
|
|
|
计价: |
|
|
|
|
|
|
|
00:00-08:00(闲时):0.125元/GB |
|
|
|
8:00-24:00(忙时):0.25元/GB |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
## 为什么我choppy的报告index.md中代码无法调用choppy report插件? |
|
|
|
|
|
|
|
调用choppy report插件的代码请勿使用代码块模式。 |
|
|
|
|
|
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## RNA-seq 报告中 multiqc所需要哪些文件? |
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- fastqc:后缀为_fastqc.zip文件 |
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- fastqscreen:后缀为_screen.txt文件 |
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- qualimap:qualimap.zip文件 |