{ | |||||
"{{ project_name }}.sample": "{{ sample }}", | |||||
"{{ project_name }}.disk_size": "150", | |||||
"{{ project_name }}.cluster_config": "OnDemand bcs.b2.3xlarge img-ubuntu-vpc", | |||||
"{{ project_name }}.docker": "registry.cn-shanghai.aliyuncs.com/pgx-docker-registry/annovar:v2018.04", | |||||
"{{ project_name }}.database": "oss://chinese-quartet/quartet-storage-data/reference_data/annovar_hg38/", | |||||
"{{ project_name }}.vcf_file": "{{ vcf }}" | |||||
} | |||||
task annovar { | |||||
File vcf_file | |||||
File database | |||||
String sample | |||||
String docker | |||||
String cluster_config | |||||
String disk_size | |||||
command <<< | |||||
nt=$(nproc) | |||||
/installations/annovar/table_annovar.pl ${vcf_file} ${database} -buildver hg38 -out ${sample} -remove -protocol refGene,ensGene,cytoBand,genomicSuperDups,ljb26_all,dbnsfp35c,intervar_20180118,cosmic70,exac03,gnomad30_genome,1000g2015aug_all,clinvar_20190305,gnomad211_genome -operation g,g,r,r,f,f,f,f,f,f,f,f,f -nastring . -vcfinput -thread $nt | |||||
>>> | |||||
runtime { | |||||
docker:docker | |||||
cluster: cluster_config | |||||
systemDisk: "cloud_ssd 40" | |||||
dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/" | |||||
} | |||||
output { | |||||
File avinput = "${sample}.avinput" | |||||
File multianno_txt = "${sample}.hg38_multianno.txt" | |||||
File multianno_vcf = "${sample}.hg38_multianno.vcf" | |||||
} | |||||
} | |||||
import "./tasks/annovar.wdl" as annovar | |||||
workflow {{ project_name }} { | |||||
File vcf_file | |||||
File database | |||||
String sample | |||||
String docker | |||||
String cluster_config | |||||
String disk_size | |||||
call annovar.annovar as annovar { | |||||
input: | |||||
vcf_file=vcf_file, | |||||
database=database, | |||||
sample=sample, | |||||
docker=docker, | |||||
cluster_config=cluster_config, | |||||
disk_size=disk_size | |||||
} | |||||
} |