prepare junction bed and gtf with novel transcripts for PGA input
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  1. task hisat2 {
  2. File idx
  3. File read1
  4. File read2
  5. String idx_prefix
  6. String sample_id
  7. String docker
  8. String cluster
  9. command {
  10. nt=$(nproc)
  11. hisat2 -t -p $nt -x ${idx}/${idx_prefix} -1 ${read1} -2 ${read2} -S ${sample_id}.sam --novel-splicesite-outfile ${sample_id}.splicesite.tab --dta
  12. }
  13. runtime {
  14. docker: docker
  15. cluster: cluster
  16. systemDisk: "cloud_ssd 40"
  17. dataDisk: "cloud_ssd 200 /cromwell_root/"
  18. }
  19. output {
  20. File sam = "${sample_id}.sam"
  21. File splicesite = "${sample_id}.splicesite.tab"
  22. }
  23. }