|
|
@@ -0,0 +1,40 @@ |
|
|
|
# DELLY Somatic Mutation Calling |
|
|
|
|
|
|
|
> Author: Ren Luyao |
|
|
|
> |
|
|
|
> E-mail:18110700050@fudan.edu.cn |
|
|
|
> |
|
|
|
> Git: http://choppy.3steps.cn/renluyao/IGV_get_bam.git |
|
|
|
> |
|
|
|
> Last Updates: 2020/0705 |
|
|
|
|
|
|
|
## 安装指南 |
|
|
|
|
|
|
|
``` |
|
|
|
# 激活choppy环境 |
|
|
|
source activate choppy |
|
|
|
# 安装app |
|
|
|
choppy install renluyao/IGV_get_bam |
|
|
|
``` |
|
|
|
|
|
|
|
## App概述 |
|
|
|
|
|
|
|
为了IGV验证突变,截取其中一段bam文件 |
|
|
|
|
|
|
|
## 流程与参数 |
|
|
|
|
|
|
|
```bash |
|
|
|
samtools view -b ${bam} "${region}" > ${region}.${sample}.bam |
|
|
|
samtools index ${region}.${sample}.bam |
|
|
|
``` |
|
|
|
|
|
|
|
samples文件中的region格式如下: |
|
|
|
|
|
|
|
```bash |
|
|
|
#chromo:start-end |
|
|
|
chr6:73900000-73920000 |
|
|
|
``` |
|
|
|
|
|
|
|
## App输出文件 |
|
|
|
|
|
|
|
输出结果为截取的bam文件和相应的bam索引文件 |