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更新 'tasks/fastqTobam.wdl'

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meng 2 years ago
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tasks/fastqTobam.wdl View File

sentieon bwa mem -R "@RG\tID:${sample_id}\tSM:${sample_id}\tPL:${Seq_platform}" ${bwa_args} -K ${bwa_chunk_size} -t $nt ${ref_fasta_dir}/${ref_fasta} ${fastq1} ${fastq2} \ sentieon bwa mem -R "@RG\tID:${sample_id}\tSM:${sample_id}\tPL:${Seq_platform}" ${bwa_args} -K ${bwa_chunk_size} -t $nt ${ref_fasta_dir}/${ref_fasta} ${fastq1} ${fastq2} \
| sentieon util sort ${bam_option} -i - -r ${ref_fasta_dir}/${ref_fasta} -t $nt -o ${sample_id}.sorted.bam --sam2bam | sentieon util sort ${bam_option} -i - -r ${ref_fasta_dir}/${ref_fasta} -t $nt -o ${sample_id}.sorted.bam --sam2bam


ls ./
sentieon driver -r ${ref_fasta_dir}/${ref_fasta} -t $nt -i ${sample_id}.sorted.bam ${qc_driver_args} \ sentieon driver -r ${ref_fasta_dir}/${ref_fasta} -t $nt -i ${sample_id}.sorted.bam ${qc_driver_args} \
--algo MeanQualityByCycle ${sample_id}.mq_metrics.txt \ --algo MeanQualityByCycle ${sample_id}.mq_metrics.txt \
--algo QualDistribution ${sample_id}.qd_metrics.txt \ --algo QualDistribution ${sample_id}.qd_metrics.txt \
--algo GCBias --summary ${sample_id}.gc_summary_metrics.txt ${sample_id}.gc_metrics.txt \ --algo GCBias --summary ${sample_id}.gc_summary_metrics.txt ${sample_id}.gc_metrics.txt \
--algo AlignmentStat ${sample_id}.aln_metrics.txt \ --algo AlignmentStat ${sample_id}.aln_metrics.txt \
--algo InsertSizeMetricAlgo ${sample_id}.is_metrics.txt --algo InsertSizeMetricAlgo ${sample_id}.is_metrics.txt
ls ./


sentieon driver -r ${ref_fasta_dir}/${ref_fasta} -t $nt -i ${sample_id}.sorted.bam ${lc_driver_args} \ sentieon driver -r ${ref_fasta_dir}/${ref_fasta} -t $nt -i ${sample_id}.sorted.bam ${lc_driver_args} \
--algo LocusCollector \ --algo LocusCollector \
${lc_args} \ ${lc_args} \
${sample_id}.score.txt.gz ${sample_id}.score.txt.gz
ls ./


sentieon driver -r ${ref_fasta_dir}/${ref_fasta} -t $nt -i ${sample_id}.sorted.bam ${dedup_driver_args} \ sentieon driver -r ${ref_fasta_dir}/${ref_fasta} -t $nt -i ${sample_id}.sorted.bam ${dedup_driver_args} \
--algo Dedup \ --algo Dedup \
--score_info ${sample_id}.score.txt.gz \ --score_info ${sample_id}.score.txt.gz \
--metrics ${sample_id}.dedup_metrics.txt \ --metrics ${sample_id}.dedup_metrics.txt \
${bam_option} ${out_bam} ${bam_option} ${out_bam}
ls ./


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