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@@ -13,31 +13,31 @@ import "./tasks/tmb.wdl" as tmb |
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#VIP客户(只用TNseq进行call) |
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workflow {{ project_name }} { |
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#sample info |
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File? normal_deduped_bam |
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File? normal_deduped_bam_bai |
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File? normal_deduped_bam |
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File? normal_deduped_bam_bai |
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File? tumor_deduped_bam |
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File? tumor_deduped_bam_bai |
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String sample_id |
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String sample_id |
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String Seq_platform |
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#reference fasta |
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String ref_fasta |
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File ref_fasta_dir |
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File annot_gff |
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File dbsnp_dir |
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File dbmills_dir |
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String dbsnp |
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String db_mills |
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File dbsnp_dir |
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File dbmills_dir |
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String dbsnp |
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String db_mills |
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File germline_resource |
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File germline_resource_tbi |
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File annovar_database |
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File? regions |
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File baseline |
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File hg38_CDS |
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File? regions |
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File baseline |
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File hg38_CDS |
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#docker |
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String docker_fastp |
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String docker_fastp |
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String docker_sentieon |
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String SENTIEON_LICENSE |
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String docker_qualimap |
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@@ -45,16 +45,16 @@ workflow {{ project_name }} { |
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String docker_strelka |
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String docker_bcftools |
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String docker_annovar |
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String docker_tmb |
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String docker_MSIsensor |
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String docker_tmb |
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String docker_MSIsensor |
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#config |
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String cluster_config |
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String disk_size |
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String cluster_config |
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String disk_size |
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Boolean? fastqc |
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Boolean bamqc |
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Boolean Annovar |
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Boolean AF_filter |
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Boolean AF_filter |
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if (normal_deduped_bam != ""){ |
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@@ -110,7 +110,7 @@ call MSIsensor.MSIsensor as MSIsensor { |
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tumor_bam_index=Sentieon_BQSR_tumor.recaled_bam_index, |
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baseline=baseline, |
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docker=docker_MSIsensor, |
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cluster_config=cluster_config, |
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cluster_config="OnDemand bcs.ps.g.large img-ubuntu-vpc", |
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disk_size=disk_size |
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} |
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@@ -137,7 +137,7 @@ call bcftools.bcftools as Haplotyper_bcftools { |
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fasta=ref_fasta, |
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ref_dir=ref_fasta_dir, |
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docker=docker_bcftools, |
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cluster_config=cluster_config, |
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cluster_config="OnDemand bcs.ps.g.large img-ubuntu-vpc", |
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disk_size=disk_size |
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} |
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@@ -170,7 +170,7 @@ call bcftools.bcftools as TNseq_bcftools { |
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fasta=ref_fasta, |
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ref_dir=ref_fasta_dir, |
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docker=docker_bcftools, |
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cluster_config=cluster_config, |
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cluster_config="OnDemand bcs.ps.g.large img-ubuntu-vpc", |
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disk_size=disk_size |
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} |
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@@ -193,7 +193,7 @@ if (Annovar){ |
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regions=regions, |
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snpindel_txt=TNseq_ANNOVAR.multianno_txt, |
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docker=docker_tmb, |
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cluster_config=cluster_config, |
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cluster_config="OnDemand bcs.ps.g.large img-ubuntu-vpc", |
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disk_size=disk_size, |
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AF_filter=AF_filter |
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} |
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@@ -206,8 +206,5 @@ if (Annovar){ |
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cluster_config=cluster_config, |
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disk_size=disk_size |
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} |
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} |
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} |