上传文件至 'tasks'

tasks/TNseq.wdl

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+task sentieon_TNseq{
+    String sample_id
+    File tumor_bam
+    File tumor_bam_bai
+    File? normal_bam
+    File? normal_bam_bai
+    String tumor_name
+    String normal_name
+
+    File ref_dir
+    String ref_fasta
+    File germline_resource
+    File germline_resource_tbi
+    File? regions
+    Int? interval_padding
+
+    # excute env
+    String docker
+    String cluster_config
+    String disk_size
+    String SENTIEON_LICENSE
+
+
+    command <<<
+        set -o pipefail
+        set -exo
+        export SENTIEON_LICENSE=${SENTIEON_LICENSE}
+        nt=$(nproc)
+
+        if [${regions}]; then
+            INTERVAL="--interval ${regions} --interval_padding ${interval_padding}"
+        else:
+            INTERVAL=""
+        fi
+
+        if [${normal_bam}];then
+            INPUT="-i ${tumor_bam} -i ${normal_bam}"
+            SAMPLE="--tumor_sample ${tumor_name} --normal_sample ${normal_name}"
+        else:
+           INPUT="-i ${tumor_bam}"
+           SAMPLE="--tumor_sample ${tumor_name}"
+        fi
+
+        sentieon driver -t $nt -r ${ref_dir}/${ref_fasta} \
+        $INPUT $INTERVAL \
+        --algo TNhaplotyper2 $SAMPLE \
+        --germline_vcf ${germline_resource} \
+        ${sample_id}.TNseq.raw.vcf \
+        --algo OrientationBias --tumor_sample ${tumor_name} \
+        ${sample_id}.orientation \
+        --algo ContaminationModel $SAMPLE \
+        --vcf ${germline_resource} \
+        --tumor_segments ${sample_id}.contamination.segments \
+        ${sample_id}.contamination
+
+        sentieon driver -t $nt \
+        -r ${ref_dir}/${ref_fasta} \
+        --algo TNfilter $SAMPLE \
+        -v ${sample_id}.TNseq.raw.vcf \
+        --contamination ${sample_id}.contamination \
+        --tumor_segments ${sample_id}.contamination.segments \
+        --orientation_priors ${sample_id}.orientation \
+        ${sample_id}.bwa_TNseq.vcf
+
+    >>>
+
+    runtime{
+        docker:docker
+        cluster:cluster_config
+        systemDisk:"cloud_ssd 40"
+        dataDisk:"cloud_ssd " + disk_size + " /cromwell_root/"
+
+    }
+
+    output{
+        File raw_vcf = "${sample_id}.TNseq.raw.vcf"
+        File raw_vcf_index = "${sample_id}.TNseq.raw.vcf.idx"
+        File vcf = "${sample_id}.bwa_TNseq.vcf"
+        File vcf_index = "${sample_id}.bwa_TNseq.vcf.idx"
+        File contamination = "${sample_id}.contamination"
+        File contamination_segments = "${sample_id}.contamination.segments"
+        File orientation = "${sample_id}.orientation"
+
+
+    }
+}