更新 'README.md'

README.md

 
 ## App输出文件
 
-
+Quality metrics
+Category
+Description
+Reference value
+Number of detected genes
+One group
+This metric is used to estimate the detection abundance of one sample. 
+(**, 58,395]
+Detection Jaccard index (JI)
+One group
+Detection JI is the ratio of number of the genes detected in both replicates than the number of the genes detected in either of the replicates. This metric is used to estimate the repeatability of one sample detected gene from different replicates. 
+[0.8, 1]
+Coefficient of variation (CV)
+One group
+CV is calculated based on the normalized expression levels in all 3 replicates of one sample for each genes. This metric is used to estimate the repeatability of one sample expression level from different replicates.
+[0, 0.2]
+Correlation of technical replicates (CTR)
+One group
+CTR is calculated based on the correlation of one sample expression level from different replicates. 
+[0.95, 1]
+Signal-to-noise Ratio (SNR)
+More groups
+Signal is defined as the average distance between libraries from the different samples on PCA plots and noise are those form the same samples. SNR is used to assess the ability to distinguish technical replicates from different biological samples.
+[5, inf)
+Sensitivity of detection
+One group
+/Reference dependent
+Sensitivity is the proportion of "true" detected genes from reference dataset which can be correctly detected by the test set. 
+[0.96, 1]
+Specificity of detection
+One group
+/Reference dependent
+Specificity is the proportion of "true" non-detected genes from reference dataset which can be correctly not detected by the test set.
+[0.94, 1]
+Consistency ratio of relative expression
+Two groups
+/Reference dependent
+Proportion of genes that falls into reference range (mean ± 2 fold SD) in relative ratio (log2FC).
+[0.82, 1]
+Correlation of relative log2FC
+Two groups
+/Reference dependent
+Pearson correlation between mean value of reference relative ratio and test site.
+[0.96,1]
+Sensitivity of DEGs
+Two groups
+/Reference dependent
+Sensitivity is the proportion of "true" DEGs from reference dataset which can be correctly identified as DEG by the test set.
+[0.80, 1]
+Specificity of DEGs
+Two groups
+/Reference dependent
+Specificity is the proportion of "true" not DEGs from reference dataset which can be can be correctly identified as non-DEG by the test set.
+[0.95, 1]