hisat2_md5/workflow.wdl

import "./tasks/fastp.wdl" as fastp
import "./tasks/hisat2.wdl" as hisat2
import "./tasks/samtools.wdl" as samtools
import "./tasks/stringtie.wdl" as stringtie
import "./tasks/fastqc.wdl" as fastqc
import "./tasks/fastqscreen.wdl" as fastqscreen
import "./tasks/qualimapBAMqc.wdl" as qualimapBAMqc
import "./tasks/qualimapRNAseq.wdl" as qualimapRNAseq
import "./tasks/ballgown.wdl" as ballgown
import "./tasks/count.wdl" as count
import "./tasks/MD5.wdl" as MD5

workflow {{ project_name }} {
	File read1
	File read2
	File idx
	File screen_ref_dir
	File fastq_screen_conf
	File gtf
	String sample_id
	String fastp_docker
	String adapter_sequence
	String adapter_sequence_r2
	String fastp_cluster
	String umi_loc
	String idx_prefix
	String pen_intronlen
	String fastqc_cluster_config
	String fastqc_docker
	String fastqscreen_docker
	String fastqscreen_cluster_config
	String hisat2_docker
	String hisat2_cluster
	String qualimapBAMqc_docker
	String qualimapBAMqc_cluster_config
	String qualimapRNAseq_docker
	String qualimapRNAseq_cluster_config
	String samtools_docker
	String samtools_cluster
	String stringtie_docker
	String stringtie_cluster
	String multiqc_cluster_config
	String multiqc_docker
	Int multiqc_disk_size
	Int trim_front1 
	Int trim_tail1 
	Int max_len1 
	Int trim_front2 
	Int trim_tail2  
	Int max_len2 
	Int disable_adapter_trimming
	Int length_required
	Int umi_len
	Int UMI
	Int qualified_quality_phred
	Int length_required1
	Int disable_quality_filtering
	Int pen_cansplice
	Int pen_noncansplice
	Int min_intronlen
	Int max_intronlen
	Int maxins
	Int minins
	Int fastqc_disk_size
	Int fastqscreen_disk_size
	Int qualimapBAMqc_disk_size
	Int qualimapRNAseq_disk_size
	Int insert_size
	Int minimum_length_allowed_for_the_predicted_transcripts
	Int Junctions_no_spliced_reads
	Int count_length
	Float minimum_isoform_abundance
	Float maximum_fraction_of_muliplelocationmapped_reads
	String ballgown_docker
	String ballgown_cluster
	String disk_size
	String count_docker
	String count_cluster


	call hisat2.hisat2 as hisat2 {
		input: 
		sample_id = sample_id, 
		idx = idx, 
		idx_prefix = idx_prefix, 
		Trim_R1 = read1, 
		Trim_R2 = read2,
		docker = hisat2_docker,
		cluster = hisat2_cluster,
		disk_size = disk_size,
		pen_intronlen = pen_intronlen,
		pen_cansplice = pen_cansplice,
		pen_noncansplice = pen_noncansplice,
		min_intronlen = min_intronlen,
		max_intronlen = max_intronlen,
		maxins = maxins,
		minins = minins
	}
		call MD5.MD5 as MD5 {
		input:
		file1 = hisat2.sam,
		file2 = hisat2.unmapread_1p,
		docker = docker,
		disk_size = disk_size,
		cluster_config=cluster_config
	}
}