@@ -0,0 +1,8 @@ | |||
{ | |||
"{{ project_name }}.cluster_config": "OnDemand bcs.a2.large img-ubuntu-vpc", | |||
"{{ project_name }}.ref_dir": "oss://pgx-reference-data/fastq_screen_reference/", | |||
"{{ project_name }}.fastq_screen_conf": "oss://pgx-reference-data/fastq_screen_reference/fastq_screen.conf", | |||
"{{ project_name }}.read": "{{ read }}", | |||
"{{ project_name }}.disk_size": "100", | |||
"{{ project_name }}.docker": "registry.cn-shanghai.aliyuncs.com/pgx-docker-registry/fastqscreen:0.12.0" | |||
} |
@@ -0,0 +1,29 @@ | |||
task fastq_screen { | |||
File read | |||
File ref_dir | |||
File fastq_screen_conf | |||
String docker | |||
String cluster_config | |||
String disk_size | |||
command <<< | |||
set -o pipefail | |||
set -e | |||
nt=$(nproc) | |||
mkdir -p /cromwell_root/tmp | |||
cp -r ${ref_dir} /cromwell_root/tmp/ | |||
fastq_screen --aligner bowtie2 --conf ${fastq_screen_conf} --threads $nt --outdir ./ ${read} | |||
>>> | |||
runtime { | |||
docker:docker | |||
cluster: cluster_config | |||
systemDisk: "cloud_ssd 40" | |||
dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/" | |||
} | |||
output { | |||
File png = sub(basename(read), "\\.(fastq|fq)\\.gz$", "_screen.html") | |||
File txt = sub(basename(read), "\\.(fastq|fq)\\.gz$", "_screen.png") | |||
File html = sub(basename(read), "\\.(fastq|fq)\\.gz$", "_screen.txt") | |||
} | |||
} |
@@ -0,0 +1,20 @@ | |||
import "./tasks/fastq_screen.wdl" as fastq_screen | |||
workflow {{ project_name }} { | |||
File read | |||
File ref_dir | |||
File fastq_screen_conf | |||
String docker | |||
String cluster_config | |||
String disk_size | |||
call fastq_screen.fastq_screen as fastq_screen { | |||
input: | |||
read=read, | |||
ref_dir=ref_dir, | |||
fastq_screen_conf=fastq_screen_conf, | |||
docker=docker, | |||
disk_size=disk_size, | |||
cluster_config=cluster_config | |||
} | |||
} |