rnaseq-engineering/workflow.wdl

import "./tasks/fastqc.wdl" as fastqc
import "./tasks/fastqscreen.wdl" as fastqscreen
import "./tasks/qualimap.wdl" as qualimap
import "./tasks/hisat2.wdl" as hisat2
import "./tasks/samtools.wdl" as samtools
import "./tasks/stringtie.wdl" as stringtie
import "./tasks/ballgown.wdl" as ballgown
import "./tasks/count.wdl" as count

workflow {{ project_name }} {

	File read1
	File read2
	File idx
	File screen_ref_dir
	File fastq_screen_conf
	String idx_prefix
	File gtf
	String disk_size
	String fastqc_docker
	String fastqc_cluster
	String fastqscreen_docker
	String fastqscreen_cluster
	String hisat2_docker
	String hisat2_cluster
	String stringtie_docker
	String stringtie_cluster
	String samtools_docker
	String samtools_cluster
	Int insert_size
	String qualimap_docker
	String qualimap_cluster
	String ballgown_docker
	String ballgown_cluster
	String count_docker
	String count_cluster
	String count_length
	String sample_id

	Boolean pre_alignment_qc
	
	if (pre_alignment_qc) {
		
		call fastqc.fastqc as fastqc {
		input:
		read1=read1,
		read2=read2,
		docker=fastqc_docker,
		cluster=fastqc_cluster,
		disk_size=disk_size
		}
		
		call fastqscreen.fastqscreen as fastqscreen {
		input:
		read1=read1,
		read2=read2,
		docker=fastqscreen_docker,
		cluster=fastqscreen_cluster,
		screen_ref_dir=screen_ref_dir,
		fastq_screen_conf=fastq_screen_conf,
		disk_size=disk_size
		}	
	}
	
	
	call hisat2.hisat2 as hisat2 {
		input: 
		docker=hisat2_docker,
		cluster=hisat2_cluster,
		idx=idx, 
		idx_prefix=idx_prefix, 
		read_1P=read1, 
		read_2P=read2,
		disk_size=disk_size
		}

	call samtools.samtools as samtools {
		input: 
		docker=samtools_docker,
		cluster=samtools_cluster,
		sam=hisat2.sam,
		insert_size = insert_size,
		disk_size=disk_size
		}

	call qualimap.qualimap as qualimap {
		input:
		bam=samtools.out_sort_bam,
		bam_percent=samtools.out_percent,
		gtf=gtf, 
		docker=qualimap_docker,
		cluster=qualimap_cluster,
		disk_size=disk_size
		}

	call stringtie.stringtie as stringtie {
		input: 
		docker=stringtie_docker,
		cluster=stringtie_cluster,
		gtf=gtf, 
		bam=samtools.out_sort_bam,
		disk_size=disk_size
		}

	call ballgown.ballgown as ballgown {
		input: 
		docker=ballgown_docker,
		cluster=ballgown_cluster,
		ballgown=stringtie.ballgown,
		gene_abundance=stringtie.gene_abundance,
		disk_size=disk_size
		} 
	
	call count.count as count {
		input: 
		sample_id=sample_id,
		docker=count_docker,
		cluster=count_cluster,
		ballgown=stringtie.ballgown,
		disk_size=disk_size,
		count_length=count_length
	}
}