rna-seq-tophat/tasks/cufflinks.wdl

task cufflinks {
    File gtf
    File bam
    File genome_directory
    String idx_prefix
    String baseout
    String docker
    String cluster

    command {
      cufflinks ${gtf} -p 24 -o ${baseout} ${bam}

    runtime {
      docker: docker 
		cluster: cluster
		systemDisk: "cloud_ssd 40"
		dataDisk: "cloud_ssd 200 /cromwell_root/"
    }
    
    output {
      File isoforms_fpkm = "${baseout}/isoforms.fpkm_tracking"
      File genes_fpkm = "${baseout}/genes.fpkm_tracking"
      File skipped_gtf = "${baseout}/skipped.gtf"
      File transcripts_gtf = "${baseout}/transcripts.gtf"
    }
}