RNA-seq pipeline with tophat2 + cufflinks + trimmomatic + fastqc
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  1. task cufflinks {
  2. File gtf
  3. File bam
  4. File accepted_hits
  5. File genome_directory
  6. String idx_prefix
  7. String baseout
  8. String docker
  9. command {
  10. cufflinks ${gtf} -p 24 -o ${baseout} ${bam}
  11. runtime {
  12. dockerTag: docker
  13. }
  14. output {
  15. File isoforms_fpkm = "${baseout}/isoforms.fpkm_tracking"
  16. File genes_fpkm = "${baseout}/genes.fpkm_tracking"
  17. File skipped_gtf = "${baseout}/skipped.gtf"
  18. File transcripts_gtf = "${baseout}/transcripts.gtf"
  19. }
  20. }