DNA-seq pipeline with Sentieon + Varscan.
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Téma musí začínat písmenem nebo číslem, může obsahovat pomlčky („-“) a může být dlouhé až 35 znaků.
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- task Dedup {
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- String SENTIEON_INSTALL_DIR
- String sample
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- File sorted_bam
- File sorted_bam_index
- String docker
- String cluster_config
- String disk_size
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- command <<<
- set -o pipefail
- set -e
- export SENTIEON_LICENSE=192.168.0.55:8990
- nt=$(nproc)
- ${SENTIEON_INSTALL_DIR}/bin/sentieon driver -t $nt -i ${sorted_bam} --algo LocusCollector --fun score_info ${sample}_score.txt
- ${SENTIEON_INSTALL_DIR}/bin/sentieon driver -t $nt -i ${sorted_bam} --algo Dedup --rmdup --score_info ${sample}_score.txt --metrics ${sample}_dedup_metrics.txt ${sample}.sorted.deduped.bam
- >>>
- runtime {
- docker:docker
- cluster: cluster_config
- systemDisk: "cloud_ssd 40"
- dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/"
- }
-
- output {
- File score = "${sample}_score.txt"
- File dedup_metrics = "${sample}_dedup_metrics.txt"
- File Dedup_bam = "${sample}.sorted.deduped.bam"
- File Dedup_bam_index = "${sample}.sorted.deduped.bam.bai"
- }
- }
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