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@@ -41,30 +41,26 @@ choppy install huangyechao/wgs-germline |
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### 流程示意图 |
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### 参数说明 |
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#### 通用参数 |
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```bash |
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-t 程序运行的线程数 |
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ref_dir 参考基因组数据所在的目录 |
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fasta 参考基因组的名字 |
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sample 生成的结果文件的前缀名 |
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-i 上一步骤的结果文件作为输入文件 |
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-o 当前步骤的输出结果文件 |
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-k Known sites |
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dbmills_dir dbmills数据所在的目录 |
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dbmills dbmills数据的名字 |
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docker task运行时使用的docker镜像 |
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cluster_config 流程运行时使用的服务器的配置 |
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disk_size 流程运行时数据盘的大小 |
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``` |
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#### 步骤参数 |
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| -t | 程序运行的线程数 | |
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| -------------- | ------------------------------ | |
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| ref_dir | 参考基因组数据所在的目录 | |
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| fasta | 参考基因组的名字 | |
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| sample | 生成的结果文件的前缀名 | |
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| -i | 上一步骤的结果文件作为输入文件 | |
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| -o | 当前步骤的输出结果文件 | |
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| -k | Known sites | |
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| dbmills_dir | dbmills数据所在的目录 | |
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| dbmills | dbmills数据的名字 | |
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| docker | task运行时使用的docker镜像 | |
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| cluster_config | 流程运行时使用的服务器的配置 | |
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| disk_size | 流程运行时数据盘的大小 | |
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步骤参数 |
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**BWA-MEM** |
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@@ -72,14 +68,14 @@ disk_size 流程运行时数据盘的大小 |
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--fastq_1 测序数据的R1端 |
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--fastq_2 测序数据的R2端 |
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-K 10000000 该设置可用于比对过程中使得结果与线程数独立 |
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-o 比对结果输出的文件名 |
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-o 比对结果输出的文件名 |
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``` |
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**Dedup** |
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```bash |
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--score_info the location and filename of the temporary score output file |
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--metrics the location and filename of the dedup metrics results output file |
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--metrics the location and filename of the dedup metrics results output file |
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``` |
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**Pre-dedup bam QC** |
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@@ -100,10 +96,10 @@ IS_METRIC_PDF the location and filename of the insertion size metrics report ou |
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**BQSR** |
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```bash |
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RECAL_DATA.TABLE the location and filename of the recalibration table |
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RECAL_DATA.TABLE.POST the location and filename of the temporary post recalibration table |
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RECAL_DATA.TABLE the location and filename of the recalibration table |
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RECAL_DATA.TABLE.POST the location and filename of the temporary post recalibration table |
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RECAL_RESULT.CSV the location and filename of the temporary recalibration results output file used for plotting |
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BQSR_PDF the location and filename of the BSQR results output file |
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BQSR_PDF the location and filename of the BSQR results output file |
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``` |
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**Haplotyper** |