{ | { | ||||
"suppa2_GTF":"oss://pgx-reference-data/reference/suppa2/gencode.v37.annotation.gtf", | |||||
"suppa2_ioe":"oss://pgx-reference-data/reference/suppa2/gencode.v37.all.events.ioe", | |||||
"salmon_index":"oss://pgx-reference-data/reference/suppa2/gencode.v37.transcripts.salmon.index/", | |||||
"suppa2_database":"oss://pgx-reference-data/reference/suppa2/", | |||||
"suppa2_docker":"registry.cn-shanghai.aliyuncs.com/pgx-docker-registry/suppa:2.3", | "suppa2_docker":"registry.cn-shanghai.aliyuncs.com/pgx-docker-registry/suppa:2.3", | ||||
"suppa2_cluster":"OnDemand bcs.b2.3xlarge img-ubuntu-vpc", | "suppa2_cluster":"OnDemand bcs.b2.3xlarge img-ubuntu-vpc", | ||||
"disk_size":"200", | "disk_size":"200", |
"{{ project_name }}.sample_id": "{{ sample_id }}", | "{{ project_name }}.sample_id": "{{ sample_id }}", | ||||
"{{ project_name }}.fastq1": "{{ fastq1 }}", | "{{ project_name }}.fastq1": "{{ fastq1 }}", | ||||
"{{ project_name }}.fastq2": "{{ fastq2 }}", | "{{ project_name }}.fastq2": "{{ fastq2 }}", | ||||
"{{ project_name }}.suppa2_GTF": "{{ suppa2_GTF }}", | |||||
"{{ project_name }}.suppa2_ioe": "{{ suppa2_ioe }}", | |||||
"{{ project_name }}.salmon_index": "{{ salmon_index }}", | |||||
"{{ project_name }}.suppa2_database": "{{ suppa2_database }}", | |||||
"{{ project_name }}.suppa2_docker": "{{ suppa2_docker }}", | "{{ project_name }}.suppa2_docker": "{{ suppa2_docker }}", | ||||
"{{ project_name }}.suppa2_cluster": "{{ suppa2_cluster }}", | "{{ project_name }}.suppa2_cluster": "{{ suppa2_cluster }}", | ||||
"{{ project_name }}.disk_size": "{{ disk_size }}", | "{{ project_name }}.disk_size": "{{ disk_size }}", |
String sample_id | String sample_id | ||||
File fastq1 | File fastq1 | ||||
File fastq2 | File fastq2 | ||||
File suppa2_GTF | |||||
File suppa2_ioe | |||||
File salmon_index | |||||
File suppa2_database | |||||
String docker | String docker | ||||
String cluster | String cluster | ||||
set -o pipefail | set -o pipefail | ||||
set -e | set -e | ||||
/software/salmon-1.5.2_linux_x86_64/bin/salmon quant -i ${salmon_index} -l ISR --gcBias -1 ${fastq1} -2 ${fastq2} -p 16 -o ./${sample_id}_salmon | |||||
/software/salmon-1.5.2_linux_x86_64/bin/salmon quant -i ${suppa2_database}/gencode.v37.transcripts.salmon.index/ -l ISR --gcBias -1 ${fastq1} -2 ${fastq2} -p 16 -o ./${sample_id}_salmon | |||||
multipleFieldSelection.py -i ./${sample_id}_salmon/quant.sf -k 1 -f 4 -o ${sample_id}_iso_tpm.txt | multipleFieldSelection.py -i ./${sample_id}_salmon/quant.sf -k 1 -f 4 -o ${sample_id}_iso_tpm.txt | ||||
sed 's@|ENSG.*|@@' ${sample_id}_iso_tpm.txt > ${sample_id}_iso_tpm_formatted.txt | sed 's@|ENSG.*|@@' ${sample_id}_iso_tpm.txt > ${sample_id}_iso_tpm_formatted.txt | ||||
suppa.py psiPerEvent -i ${suppa2_ioe} -e ${sample_id}_iso_tpm_formatted.txt -o ${sample_id}_events | |||||
suppa.py psiPerIsoform -g ${suppa2_GTF} -e ${sample_id}_iso_tpm_formatted.txt -o ${sample_id} | |||||
suppa.py psiPerEvent -i ${suppa2_database}/gencode.v37.all.events.ioe -e ${sample_id}_iso_tpm_formatted.txt -o ${sample_id}_events | |||||
suppa.py psiPerIsoform -g ${suppa2_database}/gencode.v37.annotation.gtf -e ${sample_id}_iso_tpm_formatted.txt -o ${sample_id} | |||||
>>> | >>> | ||||
String sample_id | String sample_id | ||||
File fastq1 | File fastq1 | ||||
File fastq2 | File fastq2 | ||||
File suppa2_GTF | |||||
File suppa2_ioe | |||||
File salmon_index | |||||
File suppa2_database | |||||
String suppa2_docker | String suppa2_docker | ||||
String suppa2_cluster | String suppa2_cluster | ||||
sample_id=sample_id, | sample_id=sample_id, | ||||
fastq1=fastp.Trim_R1, | fastq1=fastp.Trim_R1, | ||||
fastq2=fastp.Trim_R2, | fastq2=fastp.Trim_R2, | ||||
suppa2_GTF=suppa2_GTF, | |||||
suppa2_ioe=suppa2_ioe, | |||||
salmon_index=salmon_index, | |||||
suppa2_database=suppa2_database, | |||||
docker=suppa2_docker, | docker=suppa2_docker, | ||||
cluster=suppa2_cluster, | cluster=suppa2_cluster, | ||||
disk_size=disk_size | disk_size=disk_size |