first commit

README.md

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+word.docx

defaults

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+{   
+    "suppa2_GTF":"oss://pgx-reference-data/reference/suppa2/gencode.v37.annotation.gtf",
+    "suppa2_GTF":"oss://pgx-reference-data/reference/suppa2/gencode.v37.all.events.ioe",
+    "salmon_index":"oss://pgx-reference-data/reference/suppa2/gencode.v37.transcripts.salmon.index/"
+    "suppa2_docker":"registry.cn-shanghai.aliyuncs.com/pgx-docker-registry/suppa:2.3",
+    "suppa2_cluster":"OnDemand bcs.b2.3xlarge img-ubuntu-vpc",
+    "disk_size":"200",
+    "fastp_docker":"registry.cn-shanghai.aliyuncs.com/pgx-docker-registry/fastp:0.19.6",
+    "fastp_cluster":"OnDemand bcs.b2.3xlarge img-ubuntu-vpc",
+    "trim_front1":"0",
+    "trim_tail1":"0",
+    "max_len1":"0",
+    "trim_front2":"0",
+    "trim_tail2":"0",
+    "max_len2":"0",
+    "adapter_sequence":"AGATCGGAAGAGCACACGTCTGAACTCCAGTCA",
+    "adapter_sequence_r2":"AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT",
+    "disable_adapter_trimming":"0",
+    "length_required":"50",
+    "length_required1":"20",
+    "UMI":"0",
+    "umi_len":"0",
+    "umi_loc":"umi_loc",
+    "qualified_quality_phred":"20",
+    "disable_quality_filtering":"1"
+}

inputs

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+{
+    "{{ project_name }}.sample_id": "{{ sample_id }}",
+    "{{ project_name }}.fastq1": "{{ fastq1 }}",
+    "{{ project_name }}.fastq2": "{{ fastq2 }}",
+    "{{ project_name }}.suppa2_GTF": "{{ suppa2_GTF }}",
+    "{{ project_name }}.suppa2_ioe": "{{ suppa2_ioe }}",
+    "{{ project_name }}.salmon_index": "{{ salmon_index }}",
+    "{{ project_name }}.suppa2_docker": "{{ suppa2_docker }}",
+    "{{ project_name }}.suppa2_cluster": "{{ suppa2_cluster }}",
+    "{{ project_name }}.disk_size": "{{ disk_size }}",
+    "{{ project_name }}.fastp_docker": "{{ fastp_docker }}",
+    "{{ project_name }}.fastp_cluster": "{{ fastp_cluster }}",
+    "{{ project_name }}.trim_front1": "{{ trim_front1 }}",
+    "{{ project_name }}.trim_tail1": "{{ trim_tail1 }}",
+    "{{ project_name }}.max_len1": "{{ max_len1 }}",
+    "{{ project_name }}.trim_front2": "{{ trim_front2 }}",
+    "{{ project_name }}.trim_tail2": "{{ trim_tail2 }}",
+    "{{ project_name }}.max_len2": "{{ max_len2 }}",
+    "{{ project_name }}.adapter_sequence": "{{ adapter_sequence }}",
+    "{{ project_name }}.adapter_sequence_r2": "{{ adapter_sequence_r2 }}",
+    "{{ project_name }}.disable_adapter_trimming": "{{ disable_adapter_trimming }}",
+    "{{ project_name }}.length_required1": "{{ length_required1 }}",
+    "{{ project_name }}.UMI": "{{ UMI }}",
+    "{{ project_name }}.umi_loc": "{{ umi_loc }}",
+    "{{ project_name }}.umi_len": "{{ umi_len }}",
+    "{{ project_name }}.length_required": "{{ length_required }}",
+    "{{ project_name }}.qualified_quality_phred": "{{ qualified_quality_phred }}",
+    "{{ project_name }}.disable_quality_filtering": "{{ disable_quality_filtering }}"
+}

tasks/fastp.wdl

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+task fastp {
+    File read1
+    File read2
+    String sample_id
+    String adapter_sequence
+    String adapter_sequence_r2
+    String docker
+    String cluster
+    String disk_size
+    String umi_loc	
+    Int trim_front1
+    Int trim_tail1
+    Int max_len1
+    Int trim_front2
+    Int trim_tail2
+    Int max_len2
+    Int disable_adapter_trimming
+    Int length_required
+    Int umi_len
+    Int UMI
+    Int qualified_quality_phred
+    Int length_required1
+    Int disable_quality_filtering
+   
+	command <<<
+        mkdir -p /cromwell_root/tmp/fastp/
+	##1.Disable_quality_filtering
+	if [ "${disable_quality_filtering}" == 0 ]
+        then
+	cp ${read1} /cromwell_root/tmp/fastp/{sample_id}_R1.fastq.tmp1.gz
+	cp ${read2} /cromwell_root/tmp/fastp/{sample_id}_R2.fastq.tmp1.gz
+        else
+	fastp --thread 4 --trim_front1 ${trim_front1} --trim_tail1 ${trim_tail1} --max_len1 ${max_len1} --trim_front2 ${trim_front2} --trim_tail2 ${trim_tail2} --max_len2 ${max_len2} -i ${read1} -I ${read2} -o /cromwell_root/tmp/fastp/${sample_id}_R1.fastq.tmp1.gz -O /cromwell_root/tmp/fastp/${sample_id}_R2.fastq.tmp1.gz -j ${sample_id}.json -h ${sample_id}.html
+        fi
+
+	##2.UMI
+	if [ "${UMI}" == 0 ]
+        then
+	cp /cromwell_root/tmp/fastp/${sample_id}_R1.fastq.tmp1.gz /cromwell_root/tmp/fastp/${sample_id}_R1.fastq.tmp2.gz
+	cp /cromwell_root/tmp/fastp/${sample_id}_R2.fastq.tmp1.gz /cromwell_root/tmp/fastp/${sample_id}_R2.fastq.tmp2.gz
+        else
+	fastp --thread 4 -U --umi_loc=${umi_loc} --umi_len=${umi_len} --trim_front1 ${trim_front1} --trim_tail1 ${trim_tail1} --max_len1 ${max_len1} --trim_front2 ${trim_front2} --trim_tail2 ${trim_tail2} --max_len2 ${max_len2} -i /cromwell_root/tmp/fastp/${sample_id}_R1.fastq.tmp1.gz -I /cromwell_root/tmp/fastp/${sample_id}_R2.fastq.tmp1.gz -o /cromwell_root/tmp/fastp/${sample_id}_R1.fastq.tmp2.gz -O /cromwell_root/tmp/fastp/${sample_id}_R2.fastq.tmp2.gz -j ${sample_id}.json -h ${sample_id}.html
+	fi
+
+	##3.Trim
+        if [ "${disable_adapter_trimming}" == 0 ]
+        then
+	fastp --thread 4 -l ${length_required} -q ${qualified_quality_phred} -u ${length_required1} --adapter_sequence ${adapter_sequence} --adapter_sequence_r2 ${adapter_sequence_r2} --detect_adapter_for_pe --trim_front1 ${trim_front1} --trim_tail1 ${trim_tail1} --max_len1 ${max_len1} --trim_front2 ${trim_front2} --trim_tail2 ${trim_tail2} --max_len2 ${max_len2} -i /cromwell_root/tmp/fastp/${sample_id}_R1.fastq.tmp2.gz -I /cromwell_root/tmp/fastp/${sample_id}_R2.fastq.tmp2.gz -o ${sample_id}_R1.fastq.gz -O ${sample_id}_R2.fastq.gz -j ${sample_id}.json -h ${sample_id}.html
+        else
+	cp /cromwell_root/tmp/fastp/${sample_id}_R1.fastq.tmp2.gz ${sample_id}_R1.fastq.gz
+	cp /cromwell_root/tmp/fastp/${sample_id}_R2.fastq.tmp2.gz ${sample_id}_R2.fastq.gz
+        fi
+   >>>
+   
+   runtime { 
+		docker: docker
+		cluster: cluster
+		systemDisk: "cloud_ssd 40"
+		dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/"
+   }
+
+   output {
+      File json = "${sample_id}.json"
+      File report = "${sample_id}.html"
+      File Trim_R1 = "${sample_id}_R1.fastq.gz"
+      File Trim_R2 = "${sample_id}_R2.fastq.gz"
+   }
+}

tasks/suppa2.wdl

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+task suppa2{
+    String sample_id
+    File fastq1
+    File fastq2
+    File suppa2_GTF
+    File suppa2_ioe
+    File salmon_index
+	
+    String docker
+    String cluster
+    String disk_size
+
+    command <<<
+	
+        set -o pipefail
+        set -e
+
+        /software/salmon-1.5.2_linux_x86_64/bin/salmon quant -i ${salmon_index} -l A --gcBias -1 ${fastq1} -2 ${fastq2} -p 16 -o ./${sample_id}_salmon
+        multipleFieldSelection.py -i ./${sample_id}_salmon/quant.sf -k 1 -f 4 -o ${sample_id}_iso_tpm.txt
+        sed 's@|ENSG.*|@@' ${sample_id}_iso_tpm.txt > ${sample_id}_iso_tpm_formatted.txt
+        suppa.py psiPerEvent -i ${suppa2_ioe} -e ${sample_id}_iso_tpm_formatted.txt -o ${sample_id}_events
+        suppa.py psiPerIsoform -g ${suppa2_GTF}  -e ${sample_id}_iso_tpm_formatted.txt  -o ${sample_id}
+
+    >>>
+	
+    runtime {
+        docker : docker
+        cluster: cluster
+        systemDisk: "cloud_ssd 40"
+        dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/"
+    }
+
+    output {
+        File suppa2_events = "${sample_id}_events.psi"
+        File suppa2_isoform = "${sample_id}_events.psi"
+        File TPM = "${sample_id}_iso_tpm.txt"
+        File formatted_TPM = "${sample_id}_iso_tpm_formatted.txt"
+    }
+
+}
+
+

workflow.wdl

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+import "./tasks/suppa2.wdl" as suppa2
+import "./tasks/fastp.wdl" as fastp
+
+workflow {{ project_name }} {
+    String sample_id
+    File fastq1
+    File fastq2
+    File suppa2_GTF
+    File suppa2_ioe
+    File salmon_index
+
+    String suppa2_docker
+    String suppa2_cluster
+    String disk_size
+
+    String fastp_docker
+    String fastp_cluster
+    String adapter_sequence
+    String adapter_sequence_r2
+    String umi_loc
+    Int trim_front1
+    Int trim_tail1
+    Int max_len1
+    Int trim_front2
+    Int trim_tail2
+    Int max_len2
+    Int disable_adapter_trimming
+    Int length_required
+    Int umi_len
+    Int UMI
+    Int qualified_quality_phred
+    Int length_required1
+    Int disable_quality_filtering
+    
+        call fastp.fastp as fastp {
+                input:
+                read1=fastq1,
+                read2=fastq2,
+                sample_id=sample_id,
+                docker=fastp_docker,
+                cluster=fastp_cluster,
+                disk_size=disk_size,
+                adapter_sequence=adapter_sequence,
+                adapter_sequence_r2=adapter_sequence_r2,
+                umi_loc=umi_loc,
+                trim_front1=trim_front1,
+                trim_tail1=trim_tail1,
+                max_len1=max_len1,
+                trim_front2=trim_front2,
+                trim_tail2=trim_tail2,
+                max_len2=max_len2,
+                disable_adapter_trimming=disable_adapter_trimming,
+                length_required=length_required,
+                umi_len=umi_len,
+                UMI=UMI,
+                qualified_quality_phred=qualified_quality_phred,
+                length_required1=length_required1,
+                disable_quality_filtering=disable_quality_filtering
+        }
+
+
+
+
+
+
+
+        call suppa2.suppa2 as suppa2 {
+                input:	
+                sample_id=sample_id,
+                fastq1=fastp.Trim_R1,
+                fastq2=fastp.Trim_R2,
+                suppa2_GTF=suppa2_GTF,
+                suppa2_ioe=suppa2_ioe,
+                salmon_index=salmon_index,
+                docker=suppa2_docker,
+                cluster=suppa2_cluster,
+                disk_size=disk_size
+
+        }
+
+
+}