For RNA-seq data, from bams to AS Information files.
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- task rmats {
- String sample_id
- File bam_file
- File reference_gtf_file
-
-
- String docker
- String cluster
- String disk_size
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-
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- command <<<
- set -o pipefail
- set -e
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- mkdir -p ${sample_id}/spladder_out
-
- spladder build --bams ${bam_file} \
- --annotation ${reference_gtf_file} \
- --outdir ${sample_id}/spladder_out
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- find . -depth > fileList.txt
- >>>
-
- runtime {
- docker: docker
- cluster: cluster
- systemDisk: "cloud_ssd 40"
- dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/"
- }
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- output {
- File fileList = "fileList.txt"
- Array[File] AS_gff = glob("${sample_id}/spladder_out/*.gff3")
- Array[File] AS_pickle = glob("${sample_id}/spladder_out/*.pickle")
- Array[File] AS_gz = glob("${sample_id}/spladder_out/*.gz")
- Array[File] AS_hdf5 = glob("${sample_id}/spladder_out/*.hdf5")
- Array[File] spladder = glob("${sample_id}/spladder_out/spladder/*")
- Array[File] tmp = glob("${sample_id}/spladder_out/tmp/*")
- }
- }
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