task spladder_merge { String sample_id File bam File pickle File reference_gtf_file File samples String docker String cluster String disk_size command <<< set -o pipefail set -e mkdir -p ${sample_id}/spladder_out/spladder ln -s ${bam}/*.bam ./ cat ${samples} | while read a; do ls ./ | grep bam$ | grep $a; done > alignment.txt ln -s ${bam}/*.bai ./ cat ${samples} | while read a; do ls ./ | grep bai$ | grep $a; done > alignment_bai.txt ln -s ${pickle}/*.pickle ${sample_id}/spladder_out/spladder/ cat ${samples} | while read a; do ls ${sample_id}/spladder_out/spladder/ | grep $a; done >> pickle.txt nt=$(nproc) spladder build -o ${sample_id}/spladder_out \ --annotation ${reference_gtf_file} \ --bams alignment.txt \ --confidence 2 \ --merge-strat merge_graphs \ --readlen 150 \ --parallel $nt \ --no-extract-ase find . -depth > fileList.txt >>> runtime { docker: docker cluster: cluster systemDisk: "cloud_ssd 50" dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/" timeout: 129600 } output { File fileList = "fileList.txt" File alignment = "alignment.txt" File alignment_bai = "alignment_bai.txt" File pickle_txt = "pickle.txt" File AS_merge_pickle = "${sample_id}/spladder_out/spladder/genes_graph_conf2.merge_graphs.pickle" } }