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- task deduped_Metrics {
-
- File ref_dir
- String fasta
- File Dedup_bam
- File Dedup_bam_index
- String sample = basename(Dedup_bam,".sorted.deduped.bam")
- String docker
- String cluster_config
- String disk_size
-
-
- command <<<
- set -o pipefail
- set -e
- java -jar picard.jar CollectAlignmentSummaryMetrics /
- -I ${Dedup_bam} /
- -O ${sample}_deduped_aln_metrics.txt /
- -R ${ref_dir}/${fasta} /
- --VALIDATION_STRINGENCY LENIENT
- java -jar picard.jar CollectInsertSizeMetrics /
- -I ${Dedup_bam} /
- -O ${sample}_deduped_is_metrics.txt /
- -H ${sample}_deduped_is_metrics.pdf
- java -jar picard.jar CollectQualityYieldMetrics /
- -I ${Dedup_bam} /
- -O ${sample}_deduped_QualityYield.txt
-
- java -jar picard.jar CollectWgsMetrics
- -I ${Dedup_bam} /
- -O ${sample}_deduped_WgsMetricsAlgo.txt /
- -R ${ref_dir}/${fasta} /
- --VALIDATION_STRINGENCY LENIENT
-
- >>>
-
- runtime {
- docker:docker
- cluster: cluster_config
- systemDisk: "cloud_ssd 40"
- dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/"
- }
-
- output {
- File dedeuped_aln_metrics = "${sample}_deduped_aln_metrics.txt"
- File deduped_is_metrics = "${sample}_deduped_is_metrics.txt"
- File deduped_QualityYield = "${sample}_deduped_QualityYield.txt"
- File deduped_wgsmetrics = "${sample}_deduped_WgsMetricsAlgo.txt"
- }
- }
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