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  1. task qualimap {
  2. File bam
  3. File bai
  4. File bed
  5. String bamname = basename(bam,".bam")
  6. String docker
  7. String cluster_config
  8. String disk_size
  9. command <<<
  10. set -o pipefail
  11. set -e
  12. nt=$(nproc)
  13. awk 'BEGIN{OFS="\t"}{sub("\r","",$3);print $1,$2,$3,"",0,"."}' ${bed} > new.bed
  14. /opt/qualimap/qualimap bamqc -bam ${bam} -gff new.bed -outformat PDF:HTML -nt $nt -outdir ${bamname} --java-mem-size=60G
  15. tar -zcvf ${bamname}_qualimap.zip ${bamname}
  16. >>>
  17. runtime {
  18. docker:docker
  19. cluster:cluster_config
  20. systemDisk:"cloud_ssd 40"
  21. dataDisk:"cloud_ssd " + disk_size + " /cromwell_root/"
  22. }
  23. output {
  24. File zip = "${bamname}_qualimap.zip"
  25. }
  26. }