word.docx |
{ | |||||
"mixcr_docker":"registry.cn-shanghai.aliyuncs.com/pgx-docker-registry/mixcr:3", | |||||
"mixcr_cluster":"OnDemand bcs.b4.3xlarge img-ubuntu-vpc", | |||||
"disk_size":"200" | |||||
} |
{ | |||||
"{{ project_name }}.sample_id": "{{ sample_id }}", | |||||
"{{ project_name }}.fastq1": "{{ fastq1 }}", | |||||
"{{ project_name }}.fastq2": "{{ fastq2 }}", | |||||
"{{ project_name }}.mixcr_docker": "{{ mixcr_docker }}", | |||||
"{{ project_name }}.mixcr_cluster": "{{ mixcr_cluster }}", | |||||
"{{ project_name }}.disk_size": "{{ disk_size }}" | |||||
} |
task mixcr{ | |||||
String sample_id | |||||
File fastq1 | |||||
File fastq2 | |||||
String docker | |||||
String cluster | |||||
String disk_size | |||||
command <<< | |||||
set -o pipefail | |||||
set -e | |||||
mkdir ${sample_id}_mixcr_output | |||||
mixcr analyze shotgun --species hs \ | |||||
-t 4 \ | |||||
--starting-material rna \ | |||||
--only-productive ${fastq1} ${fastq2} ${sample_id}_mixcr_output/${sample_id} | |||||
>>> | |||||
runtime { | |||||
docker : docker | |||||
cluster: cluster | |||||
systemDisk: "cloud_ssd 40" | |||||
dataDisk: "cloud_ssd " + disk_size + " /cromwell_root/" | |||||
} | |||||
output { | |||||
Array[File] mixcr_result=glob("${sample_id}_mixcr_output/${sample_id}*") | |||||
} | |||||
} | |||||
import "./tasks/mixcr.wdl" as mixcr | |||||
workflow {{ project_name }} { | |||||
String sample_id | |||||
File fastq1 | |||||
File fastq2 | |||||
String mixcr_docker | |||||
String mixcr_cluster | |||||
String disk_size | |||||
call mixcr.mixcr as mixcr { | |||||
input: | |||||
sample_id=sample_id, | |||||
fastq1=fastq1, | |||||
fastq2=fastq2, | |||||
docker=mixcr_docker, | |||||
cluster=mixcr_cluster, | |||||
disk_size=disk_size | |||||
} | |||||
} |