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--genomeLoad NoSharedMemory \ |
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--genomeLoad NoSharedMemory \ |
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--readFilesIn ${fastq1} ${fastq2} \ |
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--readFilesIn ${fastq1} ${fastq2} \ |
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--readFilesCommand zcat \ |
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--readFilesCommand zcat \ |
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--outStd BAM_Unsorted \ |
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--outSAMtype BAM Unsorted \ |
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--outSAMtype BAM Unsorted \ |
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--outSAMunmapped Within \ |
|
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--outSAMunmapped Within \ |
|
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--outBAMcompression 0 \ |
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--outBAMcompression 0 \ |
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--chimScoreJunctionNonGTAG 0 \ |
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--chimScoreJunctionNonGTAG 0 \ |
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--chimScoreSeparation 1 \ |
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--chimScoreSeparation 1 \ |
|
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--chimSegmentReadGapMax 3 \ |
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--chimSegmentReadGapMax 3 \ |
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--chimMultimapNmax 50 > ./output/${sample_id}.Aligned.out.bam |
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--chimMultimapNmax 50 \ |
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--outFileNamePrefix ./output/${sample_id} |
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/arriba_v2.1.0/arriba \ |
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/arriba_v2.1.0/arriba \ |
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-x ./output/${sample_id}.Aligned.out.bam \ |
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-x ./output/${sample_id}Aligned.out.bam \ |
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-o ./output/${sample_id}_fusions.tsv -O ./output/${sample_id}_fusions.discarded.tsv \ |
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-o ./output/${sample_id}_fusions.tsv -O ./output/${sample_id}_fusions.discarded.tsv \ |
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-a ${ASSEMBLY_FA} \ |
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-a ${ASSEMBLY_FA} \ |
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-g ${ANNOTATION_GTF} \ |
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-g ${ANNOTATION_GTF} \ |