### NGSCheckMate - ngscheckmate_fastq A C program, ngscheckmate_fastq, can be directly called to generate a VAF file from one FASTQ file (single-end sequencing) or two FASTQ files(paired-end sequencing). ### Getting Started We recommend using choppy system and Aliyun OSS service. The command will look like this: ``` # Activate the choppy environment $ open-choppy-env # Install the APP $ choppy install YaqingLiu/ngscheckmate_fastq-latest [-f] # List the parameters $ choppy samples YaqingLiu/ngscheckmate_fastq-latest [--no-default] # Submit you task with the `samples.csv file` and `project name` $ choppy batch YaqingLiu/ngscheckmate_fastq-latest samples.csv -p Project [-l project:Label] # Query the status of all tasks in the project $ choppy query -L project:Label | grep "status" ``` #### other parameters - subsampling_rate: The default subsampling rate is 1. The speed is not very slow.