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**Author:** HGBS
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**E-mail:**16210700112@fudan.edu.cn
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**Git:** http://choppy.3steps.cn/LiXiangNan/hrd_score.git
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**Last Updates:** 23/01/2019
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**Description**
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> 本APP适用于乳腺癌WGS数据HRD score的计算,此APP需要以ascatNgs结果作为输入。
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## App使用指南
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### 安装App
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```bash
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# 激活choppy环境
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source activate choppy-latest
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# 安装app
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choppy install LiXiangNan/hrd_score
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```
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### 参数说明
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查看帮助文档:
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输入Rscript calculate_HRD.R --help
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```bash
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$ Rscript calculate_HRD.R --help
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Arguments:
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--out_dir=out_directory The output directory
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--Tumor_LogR_file=tumor_LogR The output tumor LogR file from ascatNgs
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--Tumor_BAF_file=tumor_BAF The output tumor BAF file from ascatNgs
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--Germline_LogR_file=Normal_LogR The output normal LogR file from ascatNgs
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--Germline_BAF_file=Normal_BAF The output normal BAF file from ascatNgs
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--HRD_file_prefix=prefix The name prefix of HRD score results
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--help Print help information
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Except --help, The other parameters must be set in orders!
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```
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### 输入与输出:
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输入文件为ascatNgs输出的LogR及BAF文件,如:
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```bash
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*.tumour.LogR.txt 为tumor样本LogR文件
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*.normal.LogR.txt 为normal样本LogR文件
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*.tumour.BAF.txt 为tumor样本BAF文件
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*.normal.BAF.txt 为normal样本BAF文件
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```
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输出文件后缀为.txt文件:
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```bash
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prefix.txt prfix为指定的输出文件名前缀
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```
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### 提交任务
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```bash
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choppy Rscript calculate_HRD.R --out_dir=out_directory --Tumor_LogR_file=tumor_LogR \
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--Tumor_BAF_file=tumor_BAF --Tumor_BAF_file=tumor_BAF --Germline_BAF_file=Normal_BAF \
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--Germline_BAF_file=Normal_BAF
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```
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## 更多使用信息
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详见[Choppy使用说明](http://docs.3steps.cn)) |